Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.6/3237
Título: Retinoic acid-loaded polymeric nanoparticles induce neuroprotection in a mouse model of Parkinson's disease
Autor: Esteves, Marta Raquel Carrola
Orientador: Bernardino, Liliana Inácio
Baltazar, Graça Maria Fernandes
Palavras-chave: Doença de Parkinson - Ácido retinóico
Neurónios dopaminérgicos
Neuroprotecção - Nanopartículas
Data de Defesa: Jun-2013
Resumo: Retinoic acid (RA) plays an important role in the developing mammalian nervous system and has been highlighted as a therapeutic option for some neurodegenerative diseases due to its neuroprotective, anti-inflammatory and pro-neurogenic properties. However, RA presents undesirable properties like poor water solubility and short half-life. Therefore, nanoparticles (NPs) are an excellent alternative to control the undesired side effects and to ensure intracellular transport and controlled release of RA. Thus, the aim of this work was to evaluate the effects of RA-loaded NPs (RA+-NPs) in an in vivo mouse model of Parkinson’s disease (PD) using a MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) neurotoxin, and to compare with effects of soluble RA. Interestingly, in adult mice, RA+-NPs significantly reduced the MPTP lesion by increasing the percentage of tyrosine hydroxylase positive (TH+) dopaminergic neurons in the SN to levels similar to control as well as increasing the intensity and area occupied by TH+ fibers in the striatum. This protective effect mediated by RA+-NPs was more robust than when compared with effect of soluble RA. These effects were accompanied by an increase in mRNA expression in SN and striatum of Nurr1 and Pitx3, both transcription factors involved in dopaminergic survival and specification. The same pattern of Pitx3 mRNA expression was found in the SN of old mice. In conclusion, RA+-NPs show a robust protective effect against dopaminergic injury when compared to soluble RA, suggesting that RA+-NPs could be a good strategy to boost brain repair in PD.
URI: http://hdl.handle.net/10400.6/3237
Designação: Dissertação apresentada à Universidade da Beira Interior para a obtenção do grau de Mestre em Biotecnologia
Aparece nas colecções:FC - DQ | Dissertações de Mestrado e Teses de Doutoramento

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
Dissertação_Marta Esteves_M4742.pdf934,23 kBAdobe PDFVer/Abrir

FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.