Miguel, SóniaCabral, Cátia S. D.Moreira, AndréCorreia, I.J.2019-08-262021-08-092019-09-01Sónia P. Miguel, Cátia S.D. Cabral, André F. Moreira, Ilídio J. Correia, Production and characterization of a novel asymmetric 3D printed construct aimed for skin tissue regeneration, Colloids and Surfaces B: Biointerfaces, Volume 181, 2019, Pages 994-1003, ISSN 0927-7765, https://doi.org/10.1016/j.colsurfb.2019.06.063.http://hdl.handle.net/10400.6/7179Skin is a complex organ that act as the first protective barrier against any external threat. After an injury occurs, its structure and functions must be re-established as soon as possible. Among different available skin substitutes (epidermal, dermal and dermo-epidermal), none of them is fully capable of reproducing/re-establishing all the features and functions of native skin. Herein, a three-dimensional skin asymmetric construct (3D_SAC) was produced using electrospinning and 3D bioprinting techniques. A poly(caprolactone) and silk sericin blend was electrospun to produce a top layer aimed to mimic the epidermis features, i.e. to provide a protective barrier against dehydration and hazard agents. In turn, the dermis like layer was formed by printing layer-by-layer a chitosan/sodium alginate hydrogel. The results obtained in the in vitro assays revealed that the 3D_SAC display a morphology, porosity, mechanical properties, wettability, antimicrobial activity and a cytotoxic profile that grants their application as a skin substitute during the healing process.engElectrospinning3D bioprintingAsymmetric skin constructSkin regenerationjournal article10.1016/j.colsurfb.2019.06.063