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Influenza DNA vaccine purification using pHEMA cryogel support

2018-06-02Journal article Open access
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dc.contributor.authorSantos, Tiago
dc.contributor.authorBrito, Andreia
dc.contributor.authorBoto, Renato
dc.contributor.authorSousa, Pedro
dc.contributor.authorAlmeida, Paulo
dc.contributor.authorCruz, Carla
dc.contributor.authorTomaz, C. T.
dc.date.accessioned2020-02-07T16:55:47Z
dc.date.available2022-06-02T00:30:15Z
dc.date.issued2018-06-02
dc.description.abstractInfluenza virus is a huge financial and social burden for health care systems over the world. Currently, traditionalapproaches are not effective in the fight of the epidemy and new alternatives like DNA vaccines have been developed. However, the downstream process of DNA vaccines is a constant challenge in the biotechnology industry. Cryogels has several advantages over traditional supports and have been tested as stationary phase in chromatographic separations. In this work, a method based on poly(2-hydroxyethyl methacrylate) cryogel was used to purify the plasmid NTC7482-41H-VA2 HA, which express the Influenza hemagglutinin gene. For this purpose, the cryogel was synthesized by cryo-polymerization of 2-hydroxyethyl methacrylate and characterized by scanning electron microscopy. The purification of supercoiled isoform of the plasmid NTC7482-41H-VA2 HA from a clarified lysate sample was achieved in a two-step experiment using NaCl and the dynamic binding capacity of pHEMA cryogel was determined. The assessment of DNA vaccine allowed to conclude that the level of contaminants such as proteins, genomic DNA, RNA and endotoxins are in accordance with FDA agency.pt_PT
dc.description.sponsorshipProject POCI-01-0145-FEDER-007491pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.seppur.2018.06.002pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/9147
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationNucleolin targeting by G-quadruplex aptamers for cervical cancer therapy
dc.subjectInfluenzapt_PT
dc.subjectDNA vaccinept_PT
dc.subjectpHEMA cryogelpt_PT
dc.subjectSupercoiled purificationpt_PT
dc.subjectDNA vaccine assessmentpt_PT
dc.titleInfluenza DNA vaccine purification using pHEMA cryogel supportpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNucleolin targeting by G-quadruplex aptamers for cervical cancer therapy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00959%2F2015%2FCP1300%2FCT0002/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00709%2F2013/PT
oaire.citation.endPage198pt_PT
oaire.citation.startPage192pt_PT
oaire.citation.titleSeparation and Purification Technologypt_PT
oaire.citation.volume206pt_PT
oaire.fundingStreamInvestigador FCT
oaire.fundingStream5876
person.familyNameAfonso dos Santos
person.familyNameFélix Boto
person.familyNameAlmeida
person.familyNameCruz
person.familyNameTomaz
person.givenNameTiago André
person.givenNameRenato Emanuel
person.givenNamePaulo
person.givenNameCarla
person.givenNameCândida
person.identifier2198714
person.identifier.ciencia-id6517-B7D0-2B07
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person.identifier.ciencia-idFE13-0C44-13D1
person.identifier.orcid0000-0003-4620-0174
person.identifier.orcid0000-0002-7256-2622
person.identifier.orcid0000-0003-0110-4795
person.identifier.orcid0000-0001-6630-1242
person.identifier.orcid0000-0002-0927-2331
person.identifier.ridA-1600-2014
person.identifier.ridI-7806-2013
person.identifier.ridF-9367-2014
person.identifier.scopus-author-id7102848111
person.identifier.scopus-author-id7202701394
person.identifier.scopus-author-id7004546303
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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