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Myomegalin is a novel protein of the Golgi/centrosome that interacts with a cyclic nucleotide phosphodiesterase

2001Journal article Open access
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dc.contributor.authorVerde, Ignacio
dc.contributor.authorPahlke, Gudrunpt
dc.contributor.authorSalanova, Michele
dc.contributor.authorZhang, Gupt
dc.contributor.authorWang, Sonya
dc.contributor.authorColetti, Dario
dc.contributor.authorOnuffer, James J.pt
dc.contributor.authorJin, S.-L. Catherinept
dc.contributor.authorConti, Marcopt
dc.date.accessioned2010-04-28T10:06:48Z
dc.date.available2010-04-28T10:06:48Z
dc.date.issued2001
dc.description.abstractSubcellular targeting of the components of the cAMPdependent pathway is thought to be essential for intracellular signaling. Here we have identified a novel protein, named myomegalin, that interacts with the cyclic nucleotide phosphodiesterase PDE4D, thereby targeting it to particulate structures. Myomegalin is a large 2,324-amino acid protein mostly composed of -helical and coiled-coil structures, with domains shared with microtubule-associated proteins, and a leucine zipper identical to that found in the Drosophila centrosomin. Transcripts of 7.5–8 kilobases were present in most tissues, whereas a short mRNA of 2.4 kilobases was detected only in rat testis. A third splicing variant was expressed predominantly in rat heart. Antibodies against the deduced sequence recognized particulate myomegalin proteins of 62 kDa in testis and 230–250 kDa in heart and skeletal muscle. Immunocytochemistry and transfection studies demonstrate colocalization of PDE4D and myomegalin in the Golgi/centrosomal area of cultured cells, and in sarcomeric structures of skeletal muscle. Myomegalin expressed in COS-7 cells coimmunoprecipitated with PDE4D3 and sequestered it to particulate structures. These findings indicate that myomegalin is a novel protein that functions as an anchor to localize components of the cAMP-dependent pathway to the Golgi/centrosomal region of the cell.
dc.identifier.doi10.1074/jbc.M006546200
dc.identifier.urihttp://hdl.handle.net/10400.6/553
dc.languageeng
dc.titleMyomegalin is a novel protein of the Golgi/centrosome that interacts with a cyclic nucleotide phosphodiesterasept
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue276:11189-98pt
oaire.citation.titleJournal of Biological Chemistrypt
person.familyNameVerde
person.familyNameSalanova
person.familyNameWang
person.familyNameColetti
person.givenNameIgnacio
person.givenNameMichele
person.givenNameSonya
person.givenNameDario
person.identifier.ciencia-idD012-1D7C-791A
person.identifier.orcid0000-0003-3492-5725
person.identifier.orcid0000-0002-7066-3876
person.identifier.orcid0000-0003-4429-0508
person.identifier.orcid0000-0001-7373-1953
person.identifier.ridM-3991-2013
person.identifier.scopus-author-id55913729400
person.identifier.scopus-author-id56555446800
person.identifier.scopus-author-id6701742611
rcaap.rightsopenAccess
rcaap.typearticlept
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relation.isAuthorOfPublication5ce768b7-0bc9-488d-908c-9769ba104080
relation.isAuthorOfPublication5fdd27f9-7235-4f33-b737-7999f0b29188
relation.isAuthorOfPublicationce2fc775-505c-44da-99ad-cab346a715b1
relation.isAuthorOfPublication.latestForDiscoverybfb58284-b817-4267-9628-8fa150aa681c

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