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Advisor(s)
Abstract(s)
Skin is a complex organ that act as the first protective barrier against any external threat. After an injury occurs, its structure and functions must be re-established as soon as possible. Among different available skin substitutes (epidermal, dermal and dermo-epidermal), none of them is fully capable of reproducing/re-establishing all the features and functions of native skin. Herein, a three-dimensional skin asymmetric construct (3D_SAC) was produced using electrospinning and 3D bioprinting techniques. A poly(caprolactone) and silk sericin blend was electrospun to produce a top layer aimed to mimic the epidermis features, i.e. to provide a protective barrier against dehydration and hazard agents. In turn, the dermis like layer was formed by printing layer-by-layer a chitosan/sodium alginate hydrogel. The results obtained in the in vitro assays revealed that the 3D_SAC display a morphology, porosity, mechanical properties, wettability, antimicrobial activity and a cytotoxic profile that grants their application as a skin substitute during the healing process.
Description
Keywords
Electrospinning 3D bioprinting Asymmetric skin construct Skin regeneration
Citation
Sónia P. Miguel, Cátia S.D. Cabral, André F. Moreira, Ilídio J. Correia, Production and characterization of a novel asymmetric 3D printed construct aimed for skin tissue regeneration, Colloids and Surfaces B: Biointerfaces, Volume 181, 2019, Pages 994-1003, ISSN 0927-7765, https://doi.org/10.1016/j.colsurfb.2019.06.063.
Publisher
Elsevier