Browsing by Author "Elvas, Ana Rita Gomes de"
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- Effects of dyes on candida spp. viabilityPublication . Elvas, Ana Rita Gomes de; Oliveira, José António Martinez Souto de; Oliveira, Ana Cristina Palmeira deSeveral dyes are widely recognized for their anti-microbial properties, although there spectrum of activity and security profiles are not always defined. Gentian violet (GeV) has been traditionally used to treat mucocutaneous candidosis. However, information concerning its antimicrobial spectrum required to support its clinical value is scarce. Methylene blue (MB) is widely used in clinic. Nevertheless, its antiseptic activity, although reported in the literature, is also not yet fully understood. The aim of this study is to evaluate the in vitro activity of GeV and MB against different Candida species. Nineteen strains of Candida will be studied. Clinical strains were isolated from clinical resistant cases of Candida infections. The anti-Candida activity of GeV and MB was evaluated according to CLSI protocol M27-A3. The mechanism of action it was evaluated by flow cytometry. About GeV, the minimal inhibitory concentrations (MIC) and minimal lethal concentrations (MLC) were approximately the same for all strains studied, except for C. glabrata. C. albicans and C. tropicalis were the most sensitive species. The antifungal effect was not influenced by alcohol at 20% and 96%. GeV showed a potent fungicidal activity against all strains of Candida. GeV doesn’t cause primary lesion of cytoplasmic membrane. Not all tested strains were susceptible to MB. The C. glabrata and C. krusei were resistant to the highest concentration used (5 mg/mL). In contrast to GeV, MB cause membrane lesion. Recurrent vulvovaginal candidosis, especially if resistant to classical therapeutic protocols ask for new approaches. GeV and MB continue to stand up as potential drugs to be used topically isolated or in addition to oral antifungal drugs in clinical resistant vulvovaginal candidosis.