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Percorrer por autor "Ramos, Ana Carolina da Silva"

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  • Effects of Clobetasol on Human Skin Lipids: An Ex Vivo Study - Insights into barrier disruption and regenerative potential
    Publication . Ramos, Ana Carolina da Silva; Oliveira, Rita Manuela Palmeira de; Paraskevopoulou, Anna
    The skin is the largest organ of the body, playing a vital role in the protection of the human organism. The stratum corneum (SC) is the outermost layer, composed of corneocytes embedded in a lipid matrix. Lipid ratio disruptions lead to an increase in water loss and a weaker skin barrier, causing different skin diseases. One of them, atopic dermatitis (AD), is a chronic inflammatory skin condition, impacting 15% to 20% of children and 1% to 3% of adults worldwide. As a common treatment for AD, glucocorticosteroids are used, but their transient efficacy and non-specific action often requires additional treatments, increasing the risks for adverse effects. In this project, the effect of glucocorticosteroids on the lipids of SC was investigated. To achieve this, donated human skin from plastic surgery (abdomen) was cultivated for 10 days, while applying topically clobetasol 0.05% in a suitable vehicle (propylene glycol:ethanol (7:3)) for 7 consecutive days. The viability of skin explants was tracked by the TTC assay. To gain a better understanding of the skin barrier and lipid alterations, the skin and the SC lipids were analysed using various methods, including transepidermal water loss measurement, infrared spectroscopy, alongside Liquid Chromatography with Mass Spectrometry. The results showed an increase in transepidermal water loss after treatment with clobetasol. In terms of skin viability, lipid composition, and lipid arrangement, the skin remained mostly stable. This demonstrates that short-term clobetasol treatment does not markedly alter the lipid composition of the SC. Overall, these findings help us understand how corticosteroids alter the skin barrier and SC lipids, and may inform future studies focused on creating safer, more targeted approaches to treating AD.
    2025-11-25Dissertação de mestrado Acesso embargado Ver mais