Browsing by Author "Sousa, Adriana"
Now showing 1 - 2 of 2
Results Per Page
Sort Options
- CD45RA, CD8β, and IFNγ Are Potential Immune Biomarkers of Human Cognitive FunctionPublication . Esgalhado, AJ; Reste-Ferreira, Débora; Albino, Stephanie; Sousa, Adriana; Amaral, Ana Paula; Martinho, António; Oliveira, Isabel Tomás; Verde, Ignacio; Lourenço, Olga; Fonseca, Ana M; Cardoso, Elsa M.; Arosa, FAThere is increasing evidence that in humans the adaptive immunological system can influence cognitive functions of the brain. We have undertaken a comprehensive immunological analysis of lymphocyte and monocyte populations as well as of HLA molecules expression in a cohort of elderly volunteers (age range, 64-101) differing in their cognitive status. Hereby, we report on the identification of a novel signature in cognitively impaired elderly characterized by: (1) elevated percentages of CD8+ T effector-memory cells expressing high levels of the CD45RA phosphate receptor (Temra hi); (2) high percentages of CD8+ T cells expressing high levels of the CD8β chain (CD8βhi); (3) augmented production of IFNγ by in vitro activated CD4+ T cells. Noteworthy, CD3+CD8+ Temra hi and CD3+CD8βhi cells were associated with impaired cognition. Cytomegalovirus seroprevalence showed that all volunteers studied but one were CMV positive. Finally, we show that some of these phenotypic and functional features are associated with an increased frequency of the HLA-B8 serotype, which belongs to the ancestral haplotype HLA-A1, Cw7, B8, DR3, DQ2, among cognitively impaired volunteers. To our knowledge, this is the first proof in humans linking the amount of cell surface CD45RA and CD8β chain expressed by CD8+ Temra cells, and the amount of IFNγ produced by in vitro activated CD4+ T cells, with impaired cognitive function in the elderly.
- Pedestrian Environment Quality Assessment in Portuguese Medium-Sized CitiesPublication . Sousa, Adriana; Santos, Bertha; Gonçalves, JorgeThe features of pedestrian spaces contribute to sustainable urban mobility and consequently to improve the quality of the built environment. A pedestrian space free of obstacles, with slight slopes, continuous, agreeable, attractive and safe, is definitely a space that invites to enjoyment and socialization. With the aim of improving the pedestrian circulation conditions, the present paper describes a set of approaches for assessing the quality of pedestrian environment and presents an adapted approach that can be used in medium-sized cities. Land use, pedestrian facilities and road/traffic attributes are the most common topics addressed in the pedestrian environment evaluation. These attributes can be expressed in scores and their spatial distribution can be represented using Geographical Information Systems (GIS), supporting the decision making process of pedestrian system improvement. For Portuguese medium-sized cities, the Pedestrian Environmental Quality Index (PEQI) was adapted and tested through a case study in Covilhã city. The main steps of the methodology include the definition of relevant data, preparation of survey forms, training of data collection teams, data collection, database organization, PEQI calculation and GIS data visualization, management and maps production. The case study focused on the areas that have a significant pedestrian flow, either because of commercial, services and recreation activities, or because they are important links of the public transport system. As main findings, it was possible to conclude that the selected approach allows a great flexibility in the incorporation of new attributes and weights according to specific technical goals and scenarios (calibration).