Silence is golden (siAu) - silencing the silencers via multifunctional gold nanoconjugates towards cancer therapy

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Polyurea dendrimer for efficient cytosolic siRNA delivery

Publication . Restani, Rita; Conde, João; Baptista, Pedro; Cidade, Maria Teresa Varanda; Bragança, Ana; Morgado, Jorge; Correia, Ilídio Joaquim Sobreira; Ricardo, Ana Aguiar; Bonifácio, Vasco

The design of small interfering RNA (siRNA) delivery materials showing efficacy in vivo is at the forefront of nanotherapeutics research. Polyurea (PURE-type) dendrimers are ‘smart’ biocompatible 3D polymers that unveil a dynamic and elegant back-folding mechanism involving hydrogen bonding between primary amines at the surface and tertiary amines and ureas at the core. Similarly, to a biological proton pump, they are able to automatically and reversibly transform their conformation in response to pH stimulus. Here, we show that PURE-G4 is a useful gene silencing platform showing no cellular toxicity. As a proof of concept we investigated the PURE-G4-siRNA dendriplex, which was shown to be an attractive platform with high transfection efficacy. The simplicity associated with the complexation of siRNA with polyurea dendrimers makes them a powerful tool for efficient cytosolic siRNA delivery.

2014-10-07Artigo científico

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Entidade financiadora

Fundação para a Ciência e a Tecnologia

Programa de financiamento

3599-PPCDT

Número da atribuição

PTDC/BBB-NAN/1812/2012