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Production and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regeneration

2015-05-30Journal article Restricted access
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dc.contributor.authorSerra, Inês Raquel Tavares
dc.contributor.authorFradique, Ricardo Gil
dc.contributor.authorVallejo, Mariana C. da S.
dc.contributor.authorCorreia, Tiago R.
dc.contributor.authorMiguel, Sónia P.
dc.contributor.authorCorreia, Ilídio Joaquim Sobreira
dc.date.accessioned2018-03-21T09:20:26Z
dc.date.available2018-03-21T09:20:26Z
dc.date.issued2015-05-30
dc.description.abstractRecently, bone tissue engineering emerged as a viable therapeutic alternative, comprising bone implants and new personalized scaffolds to be used in bone replacement and regeneration. In this study, biocompatible scaffolds were produced by freeze-drying, using different formulations (chitosan, chitosan/gelatin, chitosan/β-TCP and chitosan/gelatin/β-TCP) to be used as temporary templates during bone tissue regeneration. Sample characterization was performed through attenuated total reflectance-Fourier transform infrared spectroscopy, X-ray diffraction and energy dispersive spectroscopy analysis. Mechanical characterization and porosity analysis were performed through uniaxial compression test and liquid displacement method, respectively. In vitro studies were also done to evaluate the biomineralization activity and the cytotoxic profile of the scaffolds. Scanning electron and confocal microscopy analysis were used to study cell adhesion and proliferation at the scaffold surface and within their structure. Moreover, the antibacterial activity of the scaffolds was also evaluated through the agar diffusion method. Overall, the results obtained revealed that the produced scaffolds are bioactive and biocompatible, allow cell internalization and show antimicrobial activity against Staphylococcus aureus. Such, make these 3D structures as potential candidates for being used on the bone tissue regeneration, since they promote cell adhesion and proliferation and also prevent biofilm development at their surfaces, which is usually the main cause of implant failure.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSerra, I.R., Fradique, R.G., Vallejo, M.C., Correia, T.R., Miguel, S.P. e Correia I.J. (2015) "Production and characterization of Chitosan/Gelatin/β-TCP scaffolds for improved bone tissue regeneration", Materials Science & Engineering C - Materials for Biological Applications, Vol. 55, pp.592-604pt_PT
dc.identifier.doi10.1016/j.msec.2015.05.072pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4673
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationIsolation and Purification of Plasmid DNA for Cancer Therapy
dc.relationStrategic Project - UI 709 - 2011-2012
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0928493115301247pt_PT
dc.subjectAntibacterialpt_PT
dc.subjectScaffoldspt_PT
dc.subjectFreeze-dryingpt_PT
dc.subjectBiomineralization bone regenerationpt_PT
dc.subjectBiocompatibilitypt_PT
dc.titleProduction and characterization of chitosan/gelatin/β-TCP scaffolds for improved bone tissue regenerationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleIsolation and Purification of Plasmid DNA for Cancer Therapy
oaire.awardTitleStrategic Project - UI 709 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FEBB-BIO%2F114320%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FUI0709%2F2011/PT
oaire.citation.endPage604pt_PT
oaire.citation.startPage592pt_PT
oaire.citation.titleMaterials Science and Engineering: Cpt_PT
oaire.citation.volume55pt_PT
oaire.fundingStream5876-PPCDTI
oaire.fundingStream6820 - DCRRNI ID
person.familyNameFradique
person.familyNameCosta da Silva Vallejo
person.familyNameCorreia
person.familyNamePereira Miguel
person.familyNameJoaquim Sobreira Correia
person.givenNameRicardo
person.givenNameMariana
person.givenNameTiago
person.givenNameSónia Alexandra
person.givenNameIlídio
person.identifierGX0L2QAAAAJ
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-id8412-1913-C447
person.identifier.ciencia-id491D-AA5E-FBC0
person.identifier.ciencia-id1A13-ABC0-C32C
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0001-6331-2034
person.identifier.orcid0000-0002-7310-9766
person.identifier.orcid0000-0002-4077-4698
person.identifier.orcid0000-0001-7274-0399
person.identifier.orcid0000-0003-1613-9675
person.identifier.scopus-author-id55797637100
person.identifier.scopus-author-id55670188900
person.identifier.scopus-author-id55638166400
person.identifier.scopus-author-id7003557499
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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