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Evaluation of Nanoparticle Uptake in Co-culture Cancer Models

2013-07-26Journal article Restricted access
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dc.contributor.authorCosta, Elisabete C.
dc.contributor.authorGaspar, Vítor Manuel Abreu
dc.contributor.authorMarques, João Filipe Gonçalves
dc.contributor.authorCoutinho, Paula
dc.contributor.authorCorreia, Ilídio Joaquim Sobreira
dc.date.accessioned2018-03-20T08:50:52Z
dc.date.available2018-03-20T08:50:52Z
dc.date.issued2013-07-26
dc.description.abstractCo-culture models are currently bridging the gap between classical cultures and in vivo animal models. Exploring this novel approach unlocks the possibility to mimic the tumor microenvironment in vitro, through the establishment of cancer-stroma synergistic interactions. Notably, these organotypic models offer a perfect platform for the development and pre-clinical evaluation of candidate nanocarriers loaded with anti-tumoral drugs in a high throughput screening mode, with lower costs and absence of ethical issues. However, this evaluation was until now limited to co-culture systems established with precise cell ratios, not addressing the natural cell heterogeneity commonly found in different tumors. Therefore, herein the multifunctional nanocarriers efficiency was characterized in various fibroblast-MCF-7 co-culture systems containing different cell ratios, in order to unravel key design parameters that influence nanocarrier performance and the therapeutic outcome. The successful establishment of the co-culture models was confirmed by the tissue-like distribution of the different cells in culture. Nanoparticles incubation in the various co-culture systems reveals that these nanocarriers possess targeting specificity for cancer cells, indicating their suitability for being used in this illness therapy. Additionally, by using different co-culture ratios, different nanoparticle uptake profiles were obtained. These findings are of crucial importance for the future design and optimization of new drug delivery systems, since their real targeting capacity must be addressed in heterogenous cell populations, such as those found in tumors.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta, E.C., Gaspar, V.M., Marques, J.G., Coutinho, P. e Correia, I.J. (2013) "Evaluation of Nanoparticle Uptake in Co-culture Cancer Models", Plos one, Vol. 8(7):e70072pt_PT
dc.identifier.doi10.1371/journal.pone.0070072pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4637
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPLOSpt_PT
dc.relationBIOSYNTHESIS AND PURIFICATION OF MINICIRCLE DNA FOR APPLICATION IN DIABETES CELL-SPECIFIC GENE THERAPY
dc.relationIsolation and Purification of Plasmid DNA for Cancer Therapy
dc.relationStrategic Project - UI 4056 - 2011-2012
dc.relation.publisherversionhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0070072pt_PT
dc.subjectNanoparticlespt_PT
dc.subjectBionanotechnologypt_PT
dc.subjectBreast cancerpt_PT
dc.subjectFlow cytometrypt_PT
dc.subjectCell culturespt_PT
dc.subjectHistidinept_PT
dc.subjectFibroblastspt_PT
dc.subjectConfocal laser scanning microscopypt_PT
dc.titleEvaluation of Nanoparticle Uptake in Co-culture Cancer Modelspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBIOSYNTHESIS AND PURIFICATION OF MINICIRCLE DNA FOR APPLICATION IN DIABETES CELL-SPECIFIC GENE THERAPY
oaire.awardTitleIsolation and Purification of Plasmid DNA for Cancer Therapy
oaire.awardTitleStrategic Project - UI 4056 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F80402%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEME-TME%2F103375%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FEBB-BIO%2F114320%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEGE%2FUI4056%2F2011/PT
oaire.citation.startPagee70072pt_PT
oaire.citation.titlePLOS ONEpt_PT
oaire.citation.volume8pt_PT
oaire.fundingStreamFARH
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876-PPCDTI
oaire.fundingStream6817 - DCRRNI ID
person.familyNameda Rocha Costa
person.familyNameGaspar
person.familyNameTeixeira Gonçalves Coutinho Borges
person.familyNameJoaquim Sobreira Correia
person.givenNameElisabete Cristina
person.givenNameVítor
person.givenNamePaula Isabel
person.givenNameIlídio
person.identifierH-5423-2013
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-idB314-4CF0-6633
person.identifier.ciencia-id6F16-3640-73E3
person.identifier.ciencia-id2311-7DBC-FA07
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0002-0490-0095
person.identifier.orcid0000-0002-0372-2493
person.identifier.orcid0000-0001-6832-3082
person.identifier.orcid0000-0003-1613-9675
person.identifier.ridB-1602-2017
person.identifier.scopus-author-id55805850100
person.identifier.scopus-author-id36968590900
person.identifier.scopus-author-id36709479900
person.identifier.scopus-author-id7003557499
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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