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In Vitro Study of the Bioavailability and Bioaccessibility of the Main Compounds Present in Ayahuasca Beverages

2021Journal article Open access
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dc.contributor.authorGonçalves, Joana
dc.contributor.authorCastilho, Miguel
dc.contributor.authorRosado, Tiago
dc.contributor.authorLuís, Ângelo
dc.contributor.authorRestolho, José
dc.contributor.authorFernández, Nicolás
dc.contributor.authorGallardo, Eugenia
dc.contributor.authorDuarte, Ana Paula
dc.date.accessioned2021-11-08T10:10:32Z
dc.date.available2021-11-08T10:10:32Z
dc.date.issued2021
dc.description.abstractAyahuasca is a psychoactive beverage that contains the psychoactive compound N,N-dimethyltryptamine and β-carboline alkaloids. This study aims at determining in vitro the bioavailability and bioaccessibility of the main compounds present in decoctions of four individual plants, in a commercial mixture and in four mixtures of two individual plants used in the preparation of Ayahuasca. The samples were subjected to an in vitro digestion process, and the Caco-2 cell line was used as an absorption model. The integrity and permeability of the cell monolayer were evaluated, as well as the cytotoxicity of the extracts. After digestion and cell incubation, the compounds absorbed by the cell monolayer were quantified by high-performance liquid chromatography coupled to a diode array detector. The results showed that compounds such as N,N-dimethyltryptamine, Harmine, Harmaline, Harmol, Harmalol and Tetrahydroharmine were released from the matrix during the in vitro digestion process, becoming bioaccessible. Similarly, some of these compounds, after being incubated with the cell monolayer, were absorbed, becoming bioavailable. The extracts did not show cytotoxicity after cell incubation, and the integrity and permeability of the cell monolayer were not compromised.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/molecules26185555pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/11244
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationHealth Sciences Research Centre
dc.relationalterado para: “Avaliação in vitro associados ao consumo de Ayahuasca” AVALIAÇÃO IN VITRO DOS EFEITOS TOXICOLÓGICOS ASSOCIADOS AO CONSUMO DE AYAHUASCA.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAyahuascapt_PT
dc.subjectBioavailabilitypt_PT
dc.subjectBioaccessibilitypt_PT
dc.subjectPAMPApt_PT
dc.subjectHPLC-DADpt_PT
dc.titleIn Vitro Study of the Bioavailability and Bioaccessibility of the Main Compounds Present in Ayahuasca Beveragespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitlealterado para: “Avaliação in vitro associados ao consumo de Ayahuasca” AVALIAÇÃO IN VITRO DOS EFEITOS TOXICOLÓGICOS ASSOCIADOS AO CONSUMO DE AYAHUASCA.
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F149360%2F2019/PT
oaire.citation.issue18pt_PT
oaire.citation.startPage5555pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume26pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_CENTRO
person.familyNameGonçalves
person.familyNameRosado
person.familyNameSantos Luís
person.familyNameRestolho
person.familyNameFernández
person.familyNameGallardo
person.familyNameDuarte
person.givenNameJoana
person.givenNameTiago
person.givenNameÂngelo Filipe
person.givenNameJosé
person.givenNameNicolás
person.givenNameEugenia
person.givenNameAna Paula
person.identifier2664004
person.identifier908303
person.identifier1255684
person.identifier.ciencia-idC21B-DCC7-1C44
person.identifier.ciencia-id3D14-798B-65D3
person.identifier.ciencia-id5818-915B-B566
person.identifier.ciencia-idF617-5CA5-EF61
person.identifier.ciencia-idBC15-D376-80FE
person.identifier.orcid0000-0001-8702-415X
person.identifier.orcid0000-0003-0469-2895
person.identifier.orcid0000-0003-0712-6522
person.identifier.orcid0000-0002-2033-6746
person.identifier.orcid0000-0002-0980-3921
person.identifier.orcid0000-0002-1802-8998
person.identifier.orcid0000-0003-3333-5977
person.identifier.ridB-6330-2013
person.identifier.ridM-7410-2017
person.identifier.ridB-5904-2009
person.identifier.scopus-author-id57191891820
person.identifier.scopus-author-id24721485300
person.identifier.scopus-author-id7102602131
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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