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A bi-layer electrospun nanofiber membrane for plasmid DNA recovery from fermentation broths

2013-04-06Journal article Restricted access
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dc.contributor.authorCorreia, Tiago R.
dc.contributor.authorAntunes, Bernardo Paiva
dc.contributor.authorCastilho, Pedro Henrique Barata
dc.contributor.authorNunes, José
dc.contributor.authorAmorim, Maria T. Pessoa de
dc.contributor.authorEscobar, Isabel
dc.contributor.authorQueiroz, João
dc.contributor.authorCorreia, I.J.
dc.contributor.authorMorão, António
dc.date.accessioned2018-03-19T15:30:01Z
dc.date.available2018-03-19T15:30:01Z
dc.date.issued2013-04-06
dc.description.abstractThe demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. Herein we report the production of a bi-layer membrane by electrospinning (ES), in which a support of poly ε-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and were applied in the recovery process of a plasmid. The results show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. To the best of our knowledge it is the first time that ES membranes are applied in this type of bioprocess.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCorreia, T.R., Antunes, B.P., Castilho, P.H., Nunes, J.C., Amorim, M.T.P., Escobar, I. C., Queiroz, J.A., Correia, I.J. e Morão, A.M. (2013) "A bi-layer electrospun nanofiber membrane for plasmid DNA recovery from fermentation broths", Separation and Purification Technology, Vol. 112, pp. 20–25pt_PT
dc.identifier.doi10.1016/j.seppur.2013.03.049pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4636
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationIsolation and Purification of Plasmid DNA for Cancer Therapy
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1383586613001962pt_PT
dc.subjectMicrofiltrationpt_PT
dc.subjectElectrospinningpt_PT
dc.subjectBi-layer membranept_PT
dc.subjectLysatept_PT
dc.subjectPlasmid DNApt_PT
dc.titleA bi-layer electrospun nanofiber membrane for plasmid DNA recovery from fermentation brothspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleIsolation and Purification of Plasmid DNA for Cancer Therapy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEME-TME%2F103375%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FEBB-BIO%2F114320%2F2009/PT
oaire.citation.endPage25pt_PT
oaire.citation.startPage20pt_PT
oaire.citation.titleSeparation and Purification Technologypt_PT
oaire.citation.volume112pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876-PPCDTI
person.familyNameCorreia
person.familyNameAmorim
person.familyNameEscobar
person.familyNameQueiroz
person.familyNameJoaquim Sobreira Correia
person.familyNameMorão
person.givenNameTiago
person.givenNameMaria
person.givenNameIsabel
person.givenNameJoão
person.givenNameIlídio
person.givenNameAntónio
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person.identifier.orcid0000-0002-4077-4698
person.identifier.orcid0000-0003-1516-8407
person.identifier.orcid0000-0001-9269-5927
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0003-1613-9675
person.identifier.orcid0000-0001-7173-9716
person.identifier.ridO-4614-2015
person.identifier.ridL-3104-2014
person.identifier.ridI-4887-2013
person.identifier.scopus-author-id55670188900
person.identifier.scopus-author-id6506934965
person.identifier.scopus-author-id7004021627
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id7003557499
person.identifier.scopus-author-id6507774329
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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