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Estrogens down-regulate the stem cell factor (SCF)/c-KIT system in prostate cells: Evidence of antiproliferative and proapoptotic effects

2016-01-01Artigo científico Acesso restrito
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dc.contributor.authorFigueira, Marília I
dc.contributor.authorCorreia, Sara
dc.contributor.authorVaz, Cátia
dc.contributor.authorCardoso, HJ
dc.contributor.authorGomes, Inês
dc.contributor.authorMarques, Ricardo
dc.contributor.authorBaptista, Cláudio
dc.contributor.authorSocorro, Sílvia
dc.date.accessioned2019-12-20T11:59:38Z
dc.date.available2019-12-20T11:59:38Z
dc.date.issued2016-01-01
dc.description.abstractThe development of prostate cancer (PCa) is intimately associated with the hormonal environment, and the sex steroids estrogens have been implicated in prostate malignancy. However, if some studies identified estrogens as causative agents of PCa, others indicated that these steroids have a protective role counteracting prostate overgrowth. The tyrosine kinase receptor c-KIT and its ligand, the stem cell factor (SCF), have been associated with the control of cell proliferation/apoptosis and prostate carcinogenesis, and studies show that estrogens regulate their expression in different tissues, though, in the case of prostate this remains unknown. The present study aims to evaluate the role of 17β-estradiol (E2) in regulating the expression of SCF/c-KIT in human prostate cell lines and rat prostate, and to investigate the consequent effects on prostate cell proliferation and apoptosis. qPCR, Western Blot, and immuno(cito)histochemistry analysis showed that E2-treatment decreased the expression of SCF and c-KIT both in human prostate cells and rat prostate. Furthermore, the diminished expression of SCF/c-KIT was underpinned by the diminished prostate weight and reduced proliferation index. On the other hand, the results of TUNEL labelling, the increased activity of caspase-3, and the augmented expression of caspase-8 and Fas system in the prostate of E2-treated animals indicated augmented apoptosis in response to E2. The obtained results demonstrated that E2 down-regulated the expression of SCF/c-KIT system in prostate cells, which was associated with antiproliferative and proapoptotic effects. Moreover, these findings support the protective role of estrogens in PCa and open new perspectives on the application of estrogen-based therapies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.bcp.2015.11.016pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/7999
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationProjeto Estratégico - UI 709 - 2014 (Strategic Project - UI 709 - 2014): CICS-UBI [PEst-OE/SAU/UI0709/2014]
dc.relationBolsa de Doutoramento FCT: Estrogens and prostate cancer: role of GPER and regulation of stem cell factor SCF/c-KIT system [SFRH/BD/104671/2014]
dc.relationBolsa de Pós-Doutoramento FCT: Effect of endocrine disruptors on spermatogenesis and testicular cancer: where does the protective role of regucalcin fit in [SFRH/BPD/104820/2014]
dc.relationROLE OF REGUCALCIN IN BREAST CELLS PROLIFERATION AND TUMOR DEVELOPMENT
dc.relationBolsa de Doutoramento FCT: Androgens/anti-androgens and glycaemia in reprogramming metabolism of prostate cancer: targeting both androgen receptor and metabolism as a therapeutic option [SFRH/BD/111351/2015]
dc.relationBolsa de doutoramento FCT: Papel da proteína regucalcina na proliferação e apoptose celular, particularmente no contexto do cancro da mama e da próstata [SFRH/BD/66875/2009]
dc.subjectApoptosispt_PT
dc.subjectEstrogenspt_PT
dc.subjectProliferationpt_PT
dc.subjectProstate cancerpt_PT
dc.subjectSCFpt_PT
dc.subjectC-KITpt_PT
dc.titleEstrogens down-regulate the stem cell factor (SCF)/c-KIT system in prostate cells: Evidence of antiproliferative and proapoptotic effectspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberPEst-OE/SAU/UI0709/2014
oaire.awardNumberSFRH/BD/104671/2014
oaire.awardNumberSFRH/BPD/104820/2014
oaire.awardNumberSFRH/BD/70316/2010
oaire.awardNumberSFRH/BD/111351/2015
oaire.awardNumberSFRH/BD/66875/2009
oaire.awardTitleProjeto Estratégico - UI 709 - 2014 (Strategic Project - UI 709 - 2014): CICS-UBI [PEst-OE/SAU/UI0709/2014]
oaire.awardTitleBolsa de Doutoramento FCT: Estrogens and prostate cancer: role of GPER and regulation of stem cell factor SCF/c-KIT system [SFRH/BD/104671/2014]
oaire.awardTitleBolsa de Pós-Doutoramento FCT: Effect of endocrine disruptors on spermatogenesis and testicular cancer: where does the protective role of regucalcin fit in [SFRH/BPD/104820/2014]
oaire.awardTitleROLE OF REGUCALCIN IN BREAST CELLS PROLIFERATION AND TUMOR DEVELOPMENT
oaire.awardTitleBolsa de Doutoramento FCT: Androgens/anti-androgens and glycaemia in reprogramming metabolism of prostate cancer: targeting both androgen receptor and metabolism as a therapeutic option [SFRH/BD/111351/2015]
oaire.awardTitleBolsa de doutoramento FCT: Papel da proteína regucalcina na proliferação e apoptose celular, particularmente no contexto do cancro da mama e da próstata [SFRH/BD/66875/2009]
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FSAU%2FUI0709%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F104671%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F104820%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F70316%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F111351%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F66875%2F2009/PT
oaire.citation.endPage87pt_PT
oaire.citation.startPage73pt_PT
oaire.citation.titleBiochemical Pharmacologypt_PT
oaire.citation.volume99pt_PT
oaire.fundingStream5876
oaire.fundingStreamBolsa de Doutoramento
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person.givenNameSara
person.givenNameCátia
person.givenNameHenrique
person.givenNameInês
person.givenNameCláudio Jorge
person.givenNameSílvia Cristina da Cruz Marques
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rcaap.embargofctCopyright cedido à editora no momento da publicação.pt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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