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Sensitive Detection of Peptide–Minicircle DNA Interactions by Surface Plasmon Resonance

2013-01-22Journal article Restricted access
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dc.contributor.authorGaspar, Vítor Manuel Abreu
dc.contributor.authorCruz, Carla Patrícia Alves Freire Madeira
dc.contributor.authorQueiroz, João
dc.contributor.authorPichon, Chantal
dc.contributor.authorCorreia, Ilídio
dc.contributor.authorSousa, Fani
dc.date.accessioned2018-03-20T09:19:15Z
dc.date.available2018-03-20T09:19:15Z
dc.date.issued2013-01-22
dc.description.abstractMinicircle DNA (mcDNA) is recently becoming an exciting source of genetic material for therapeutic purposes due to its exceptional biocompatibility and efficiency over typical DNA. However, its widespread use is yet restrained because of the absence of an efficient technology that allows its purification. Here, the precise conditions of mcDNA interaction with novel arginine-arginine dipeptide ligands were explored to promote binding and recovery of these biopharmaceuticals. Such interactions were investigated by taking advantage of a highly sensitive method based on surface plasmon resonance (SPR) to screen, in real-time, for ligand-coupled biomolecules, while preserving mcDNA integrity. Through this analytic approach, we detected dynamic binding responses that are dependent on buffer type, mcDNA electrokinetic potential, and temperature conditions. Remarkably, the results obtained revealed that the ligands possess high affinity to mcDNA molecules under low salt buffers, and low affinity in the presence of salt, suggesting that electrostatic interactions mainly govern ligand–analyte coupling. These findings provide important insights for an active manipulation of parameters that promote mcDNA recovery and purification. Above all, this study showed the crucial importance of SPR for future screening of other ligands that, like the one described herein, can be used to design mcDNA recovery platforms which will have significant impact in biopharmaceutical-based therapeutics.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGaspar, V.M., Cruz, C., Queiroz, J.A., Pichon, C., Correia, I.J. e Sousa, F. (2013) “Sensitive detection of peptide - minicircle DNA interactions by Surface Plasmon Resonance”, Analytical Chemistry, Vol. 85(4), pp. 2304-11pt_PT
dc.identifier.doi10.1021/ac303288xpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4640
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Societypt_PT
dc.relationIsolation and Purification of Plasmid DNA for Cancer Therapy
dc.relationStrategic Project - UI 709 - 2011-2012
dc.relationBIOSYNTHESIS AND PURIFICATION OF MINICIRCLE DNA FOR APPLICATION IN DIABETES CELL-SPECIFIC GENE THERAPY
dc.relationESTUDOS DE LIGAÇÃO ENTRE OLIGONUCLEÓTIDOS E AMINOÁCIDOS POR RESSONÂNCIA DE PLASMA DE SUPERFÍCIE E RESSONÂNCIA MAGNÉTICA NUCLEAR
dc.relation.publisherversionhttps://pubs.acs.org/doi/abs/10.1021/ac303288xpt_PT
dc.subjectMinicircle DNApt_PT
dc.subjectPurificationpt_PT
dc.subjectSurface Plasmon Resonancept_PT
dc.titleSensitive Detection of Peptide–Minicircle DNA Interactions by Surface Plasmon Resonancept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleIsolation and Purification of Plasmid DNA for Cancer Therapy
oaire.awardTitleStrategic Project - UI 709 - 2011-2012
oaire.awardTitleBIOSYNTHESIS AND PURIFICATION OF MINICIRCLE DNA FOR APPLICATION IN DIABETES CELL-SPECIFIC GENE THERAPY
oaire.awardTitleESTUDOS DE LIGAÇÃO ENTRE OLIGONUCLEÓTIDOS E AMINOÁCIDOS POR RESSONÂNCIA DE PLASMA DE SUPERFÍCIE E RESSONÂNCIA MAGNÉTICA NUCLEAR
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEME-TME%2F103375%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FEBB-BIO%2F114320%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FUI0709%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F80402%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F46934%2F2008/PT
oaire.citation.endPage2311pt_PT
oaire.citation.startPage2304pt_PT
oaire.citation.titleAnalytical Chemistrypt_PT
oaire.citation.volume85pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876-PPCDTI
oaire.fundingStream6820 - DCRRNI ID
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person.familyNameGaspar
person.familyNameQueiroz
person.familyNameJoaquim Sobreira Correia
person.familyNameSousa
person.givenNameVítor
person.givenNameJoão
person.givenNameIlídio
person.givenNameFani
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person.identifier.orcid0000-0002-0372-2493
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0003-1613-9675
person.identifier.orcid0000-0001-9996-2194
person.identifier.ridB-1602-2017
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project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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