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Glucose and glutamine handling in the Sertoli cells of transgenic rats overexpressing regucalcin: plasticity towards lactate production

2018-07-09Journal article Open access
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dc.contributor.authorMateus, Inês
dc.contributor.authorFeijó, Mariana
dc.contributor.authorEspínola, Luís M
dc.contributor.authorVaz, CV
dc.contributor.authorCorreia, Sara
dc.contributor.authorSocorro, Sílvia
dc.date.accessioned2018-07-18T09:05:48Z
dc.date.available2018-07-18T09:05:48Z
dc.date.issued2018-07-09
dc.description.abstractSertoli cells (SCs) possess the unparalleled ability to provide the germ line with growth factors and nutrients. Although SCs can oxidize amino acids, e.g., glutamine, they mostly metabolize glucose, producing high amounts of lactate, the germ cells preferential substrate. Regucalcin (RGN) is a calcium-binding protein that has been indicated as a regulator of cell metabolism. In this study, we investigated glucose and glutamine handling in the SCs of transgenic rats overexpressing RGN (Tg-RGN) comparatively with wild-type (Wt) littermates. Primary SCs isolated from adult Tg-RGN animals and maintained in culture for 24 hours, produced and exported more lactate, despite consuming less glucose. These observations were underpinned by increased expression of alanine transaminase, and augmented glutamine consumption, suggesting that alternative routes are contributing to the enhanced lactate production in the SCs of Tg-RGN rats. Moreover, lactate seems to be used by germ cells, with diminished apoptosis being detected in the seminiferous tubules of Tg-RGN animals cultured ex vivo. The obtained results showed a distinct metabolism in the SCs of Wt and Tg-RGN rats widening the roles assigned to RGN in spermatogenesis. These findings also highlighted the plasticity of SCs metabolism, a feature that would be exploited in the context of male infertility.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1038/s41598-018-28668-4pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/5181
dc.language.isoengpt_PT
dc.subjectSertoli Cellspt_PT
dc.subjectMetabolismpt_PT
dc.subjectRegucalcinpt_PT
dc.subjectGlucosept_PT
dc.subjectGlutaminept_PT
dc.titleGlucose and glutamine handling in the Sertoli cells of transgenic rats overexpressing regucalcin: plasticity towards lactate productionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.startPage10321pt_PT
oaire.citation.volume8pt_PT
person.familyNameVaz
person.familyNameCorreia
person.familyNameSocorro
person.givenNameCátia
person.givenNameSara
person.givenNameSílvia Cristina da Cruz Marques
person.identifierhttps://scholar.google.pt/citations?user=PA3L_h8AAAAJ&hl=pt-PT
person.identifier.ciencia-id1C1D-F91F-D937
person.identifier.ciencia-id7518-7FBC-A42F
person.identifier.ciencia-idAB17-5C25-1F2C
person.identifier.orcid0000-0002-7720-4234
person.identifier.orcid0000-0001-9199-8360
person.identifier.orcid0000-0001-8181-488X
person.identifier.scopus-author-id7004157751
person.identifier.scopus-author-id23097994600
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationeaf1f3ee-dc44-441b-bbfd-b51e72fcab80
relation.isAuthorOfPublication049d1335-73d4-46c8-a99d-b96d9e996f17
relation.isAuthorOfPublication1fb06949-0e6c-41a6-bb25-11885e7f0e9a
relation.isAuthorOfPublication.latestForDiscovery1fb06949-0e6c-41a6-bb25-11885e7f0e9a

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