Repository logo
 
Publication

Comparative study of the therapeutic effect of Doxorubicin and Resveratrol combination on 2D and 3D (spheroids) cell culture models

2018-09-11Journal article Restricted access
Simple item page
dc.contributor.authorBarros, Andreia
dc.contributor.authorCosta, Elisabete C.
dc.contributor.authorNunes, Ana Raquel Santos
dc.contributor.authorDiogo, Duarte Miguel de Melo
dc.contributor.authorCorreia, Ilídio Joaquim Sobreira
dc.date.accessioned2018-09-24T14:51:46Z
dc.date.available2018-09-24T14:51:46Z
dc.date.issued2018-09-11
dc.description.abstractThe assessment of drug-combinations for pancreatic cancer treatment is usually performed in 2D cell cultures. In this study, the therapeutic effect and the synergistic potential of a particular drug-combination towards 2D and 3D cell cultures of pancreatic cancer were compared for the first time. Thus, the effect of Doxorubicin:Resveratrol (DOX:RES) combinations (at molar ratios ranging from 5:1 to 1:5) in the viability of PANC-1 cells cultured as 2D monolayers and as 3D spheroids was analyzed. The results showed that the cells’ viability was more affected when DOX:RES combinations containing higher contents of RES (1:2–1:5 molar ratios) were used. This can be explained by the ability of RES to reduce the P-glycoprotein (P-gp)-mediated efflux of DOX. Further, it was also revealed that the synergic effect of this drug combination was different in 2D and in 3D cell cultures. In fact, despite of the 1:4 and 1:5 DOX:RES ratios being both synergistic for both types of PANC-1 cell cultures, their Combination Indexes (CI) in the monolayers were lower than those attained in spheroids. Overall, the obtained results revealed that the DOX:RES combination is promising for pancreatic cancer treatment and corroborate the emergent need to evaluate drug combinations in 3D cell cultures.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBarros, A.S., Costa, E.C., Nunes, A.S., de Melo-Diogo, D., Correia, I.J. (2018) "Comparative study of the therapeutic effect of Doxorubicin and Resveratrol combination on 2D and 3D (spheroids) cell cultures models", International Journal of Pharmaceutics, Vol. 551, 76-83.pt_PT
dc.identifier.doi10.1016/j.ijpharm.2018.09.016pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/6243
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationHealth Sciences Research Centre
dc.relation3D multicellular spheroids as high throughput platforms to screen novel combinatory therapies for treatment of pancreatic cancer
dc.relationMULTIFUNCTIONAL GRAPHENE-OXIDE NANOCARRIERS FOR DRUG DELIVERY AND PHOTOTHERMAL THERAPY OF LUNG CANCER
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0378517318306677pt_PT
dc.subject2D cell culturespt_PT
dc.subjectSpheroidspt_PT
dc.subjectPancreatic cancerpt_PT
dc.subjectResveratrolpt_PT
dc.subjectDoxorubicinpt_PT
dc.titleComparative study of the therapeutic effect of Doxorubicin and Resveratrol combination on 2D and 3D (spheroids) cell culture modelspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitle3D multicellular spheroids as high throughput platforms to screen novel combinatory therapies for treatment of pancreatic cancer
oaire.awardTitleMULTIFUNCTIONAL GRAPHENE-OXIDE NANOCARRIERS FOR DRUG DELIVERY AND PHOTOTHERMAL THERAPY OF LUNG CANCER
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F00709%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F103507%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F103506%2F2014/PT
oaire.citation.endPage83pt_PT
oaire.citation.startPage76pt_PT
oaire.citation.titleInternational Journal of Pharmaceuticspt_PT
oaire.citation.volume551pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSousa Barros
person.familyNameda Rocha Costa
person.familyNameMiguel de Melo Diogo
person.familyNameJoaquim Sobreira Correia
person.givenNameAndreia Sofia
person.givenNameElisabete Cristina
person.givenNameDuarte
person.givenNameIlídio
person.identifierC2z5x04AAAAJ
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-idC213-C723-4528
person.identifier.ciencia-idB314-4CF0-6633
person.identifier.ciencia-id9C10-9BD8-634E
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0002-0522-1705
person.identifier.orcid0000-0002-0490-0095
person.identifier.orcid0000-0001-9984-3603
person.identifier.orcid0000-0003-1613-9675
person.identifier.scopus-author-id55805850100
person.identifier.scopus-author-id56266511300
person.identifier.scopus-author-id7003557499
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3f895ebf-cc75-46fd-9357-31fd5e104503
relation.isAuthorOfPublicationbfc28f1b-bd1b-4564-97e3-ae6a0c3b6c0c
relation.isAuthorOfPublication8525f1be-ff8f-4e46-b158-e59928e8a3e5
relation.isAuthorOfPublicationf2c3e779-34a2-4309-950a-80687664c6ad
relation.isAuthorOfPublication.latestForDiscoverybfc28f1b-bd1b-4564-97e3-ae6a0c3b6c0c
relation.isProjectOfPublication1bea56d9-b91c-42af-a6d6-8004792eaac0
relation.isProjectOfPublicationf7269f43-1c1e-4809-9b13-8bf68efc42c6
relation.isProjectOfPublicationa61e1cb2-bd08-4ddf-806d-76b237068ee3
relation.isProjectOfPublication.latestForDiscovery1bea56d9-b91c-42af-a6d6-8004792eaac0

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
2018 Barros IJP.pdf
Size:
1.93 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

FCS - DCM | Documentos por Auto-Depósito