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Haemodialysis in Diabetic Patients Modulates Inflammatory Cytokine Profile and T Cell Activation Status

2015-08Journal article Open access
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dc.contributor.authorAlmeida, Ana Catarina Silva
dc.contributor.authorLourenço, Olga
dc.contributor.authorFonseca, A. M.
dc.date.accessioned2020-02-18T17:22:20Z
dc.date.available2020-02-18T17:22:20Z
dc.date.issued2015-08
dc.description.abstractDiabetic nephropathy (DN) is a common complication in patients with diabetes, and most of them need renal replacement therapy such as haemodialysis (HD). These patients have a high tendency to develop infections and exhibit anomalies in the immune system. The objective of this study was to assess the expression of activation-related markers on T cells, as well as to quantify inflammatory cytokines, before and after a single HD session in DN patients. The study involved DN patients under HD treatment who signed an informed consent form. Blood samples before and after one HD session were collected, to analyse the expression of CD25, CD69 and CD71 in T cells. We also quantified IL-12p70, IL-8, IL-10, IL-1β, TNF-α and IL-6 in serum samples using the cytometric bead array technique. After the HD session, there was an increase in the CD4/CD8 ratio due to significant alterations in both subsets. The relative percentage of CD25+ cells and CD8+ CD25+ increased significantly after the HD session, while the relative percentage of CD69 T cells decreased. There was a significant decrease in the CD25 mean fluorescence intensity values for CD4+ T, as well as in the case of CD71 in T cells after the HD session. Regarding cytokine synthesis, we found a significant increase in IL-10 and IL-6 and a decrease in IL-8 after HD session. This study showed that a HD session in DN patients affects the T cell activation status in the two major subpopulations and differentially modulates the production of inflammatory cytokines.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1111/sji.12309pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/9354
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectAgedpt_PT
dc.subjectAntigens, CDpt_PT
dc.subjectCD4-CD8 Ratiopt_PT
dc.subjectCytokinespt_PT
dc.subjectDiabetes Mellituspt_PT
dc.subjectDiabetic Nephropathiespt_PT
dc.subjectFemalept_PT
dc.subjectHumanspt_PT
dc.subjectLymphocyte Activationpt_PT
dc.subjectMalept_PT
dc.subjectRenal Dialysispt_PT
dc.subjectT-Lymphocytespt_PT
dc.titleHaemodialysis in Diabetic Patients Modulates Inflammatory Cytokine Profile and T Cell Activation Statuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FSAU%2FUI0709%2F2014/PT
oaire.citation.endPage141pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage135pt_PT
oaire.citation.titleScandinavian Journal of Immunologypt_PT
oaire.citation.volume82pt_PT
oaire.fundingStream5876
person.familyNameLourenço
person.familyNameLoureiro Fonseca
person.givenNameOlga Maria Marques
person.givenNameAna Mafalda
person.identifierH-3836-2012
person.identifier.ciencia-id6618-1C4C-D1B7
person.identifier.ciencia-idA61D-657A-6DD0
person.identifier.orcid0000-0002-8401-5976
person.identifier.orcid0000-0003-1118-5525
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationc890c74a-abc1-4910-aa70-f0da7b4ad902
relation.isAuthorOfPublication34cfbd2d-5537-4aba-8552-3e2e4d48b473
relation.isAuthorOfPublication.latestForDiscovery34cfbd2d-5537-4aba-8552-3e2e4d48b473
relation.isProjectOfPublicationf6d0aa1d-d8a8-4438-b222-023db42ef45e
relation.isProjectOfPublication.latestForDiscoveryf6d0aa1d-d8a8-4438-b222-023db42ef45e

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