Publication
3D tumor spheroids: an overview on the tools and techniques used for their analysis
dc.contributor.author | Costa, Elisabete C. | |
dc.contributor.author | Moreira, André | |
dc.contributor.author | Diogo, Duarte Miguel de Melo | |
dc.contributor.author | Gaspar, V. M. | |
dc.contributor.author | Carvalho, Marco António Paulo de | |
dc.contributor.author | Correia, I.J. | |
dc.date.accessioned | 2018-03-21T15:40:51Z | |
dc.date.available | 2018-03-21T15:40:51Z | |
dc.date.issued | 2016-11-11 | |
dc.description.abstract | In comparison with 2D cell culture models, 3D spheroids are able to accurately mimic some features of solid tumors, such as their spatial architecture, physiological responses, secretion of soluble mediators, gene expression patterns and drug resistance mechanisms. These unique characteristics highlight the potential of 3D cellular aggregates to be used as in vitro models for screening new anticancer therapeutics, both at a small and large scale. Nevertheless, few reports have focused on describing the tools and techniques currently available to extract significant biological data from these models. Such information will be fundamental to drug and therapeutic discovery process using 3D cell culture models. The present review provides an overview of the techniques that can be employed to characterize and evaluate the efficacy of anticancer therapeutics in 3D tumor spheroids. | pt_PT |
dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
dc.identifier.doi | 10.1016/j.biotechadv.2016.11.002 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10400.6/4690 | |
dc.language.iso | eng | pt_PT |
dc.peerreviewed | yes | pt_PT |
dc.publisher | Elsevier | pt_PT |
dc.relation | Health Sciences Research Centre | |
dc.relation | 3D multicellular spheroids as high throughput platforms to screen novel combinatory therapies for treatment of pancreatic cancer | |
dc.relation | Development of layer-by-layer polymeric microneedles for localized delivery of therapeutics to cervical cancer | |
dc.relation | MULTIFUNCTIONAL GRAPHENE-OXIDE NANOCARRIERS FOR DRUG DELIVERY AND PHOTOTHERMAL THERAPY OF LUNG CANCER | |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0734975016301379 | pt_PT |
dc.subject | 3D tumor spheroids | pt_PT |
dc.subject | Cancer therapy | pt_PT |
dc.subject | In vitro models | pt_PT |
dc.subject | Methodologies | pt_PT |
dc.subject | Therapeutic screening | pt_PT |
dc.title | 3D tumor spheroids: an overview on the tools and techniques used for their analysis | pt_PT |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.awardTitle | Health Sciences Research Centre | |
oaire.awardTitle | 3D multicellular spheroids as high throughput platforms to screen novel combinatory therapies for treatment of pancreatic cancer | |
oaire.awardTitle | Development of layer-by-layer polymeric microneedles for localized delivery of therapeutics to cervical cancer | |
oaire.awardTitle | MULTIFUNCTIONAL GRAPHENE-OXIDE NANOCARRIERS FOR DRUG DELIVERY AND PHOTOTHERMAL THERAPY OF LUNG CANCER | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F00709%2F2013/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F103507%2F2014/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F109482%2F2015/PT | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F103506%2F2014/PT | |
oaire.citation.endPage | 1441 | pt_PT |
oaire.citation.startPage | 1427 | pt_PT |
oaire.citation.title | Biotechnology Advances | pt_PT |
oaire.citation.volume | 34 | pt_PT |
oaire.fundingStream | 6817 - DCRRNI ID | |
oaire.fundingStream | POR_CENTRO | |
person.familyName | da Rocha Costa | |
person.familyName | Moreira | |
person.familyName | Miguel de Melo Diogo | |
person.familyName | Gaspar | |
person.familyName | Joaquim Sobreira Correia | |
person.givenName | Elisabete Cristina | |
person.givenName | André Ferreira | |
person.givenName | Duarte | |
person.givenName | Vítor | |
person.givenName | Ilídio | |
person.identifier | 1161643 | |
person.identifier | C2z5x04AAAAJ | |
person.identifier | UMbJ1KMAAAAJ | |
person.identifier.ciencia-id | B314-4CF0-6633 | |
person.identifier.ciencia-id | 4C1E-012B-83CE | |
person.identifier.ciencia-id | 9C10-9BD8-634E | |
person.identifier.ciencia-id | 6F16-3640-73E3 | |
person.identifier.ciencia-id | F610-7373-DC81 | |
person.identifier.orcid | 0000-0002-0490-0095 | |
person.identifier.orcid | 0000-0002-0604-2506 | |
person.identifier.orcid | 0000-0001-9984-3603 | |
person.identifier.orcid | 0000-0002-0372-2493 | |
person.identifier.orcid | 0000-0003-1613-9675 | |
person.identifier.rid | B-1602-2017 | |
person.identifier.scopus-author-id | 55805850100 | |
person.identifier.scopus-author-id | 56401521300 | |
person.identifier.scopus-author-id | 56266511300 | |
person.identifier.scopus-author-id | 36968590900 | |
person.identifier.scopus-author-id | 7003557499 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
rcaap.embargofct | Copyright cedido à editora no momento da publicação | pt_PT |
rcaap.rights | closedAccess | pt_PT |
rcaap.type | article | pt_PT |
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