Synergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymers

Cunha, M. N. M.; Felgueiras, Helena Prado; Gouveia, Isabel C.; Andrea, Zille

Publication

Synergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymers

2017Journal article

dc.contributor.author	Cunha, M. N. M.
dc.contributor.author	Felgueiras, Helena Prado
dc.contributor.author	Gouveia, Isabel C.
dc.contributor.author	Andrea, Zille
dc.date.accessioned	2019-11-04T12:04:29Z
dc.date.available	2019-11-04T12:04:29Z
dc.date.issued	2017
dc.description.abstract	Silver nanoparticles (AgNPs) were synthesized by citrate reduction method in the presence of polymers, poly(ethylene glycol) (PEG), poly(vinyl alcohol) (PVA) and chitosan, used as stabilizing agents, and an oxidoreductase enzyme, laccase (Lac), with the goal of expanding the NPs antimicrobial action. AgNPs were characterized by UV-vis spectrometry, dynamic light scattering and transmission electron microscopy. As protecting agents, PEG and PVA promoted the formation of spherical uniformly-shaped, small-sized, monodispersed AgNPs (≈20nm). High Mw polymers were established as most effective in producing small-sized NPs. Chitosan's viscosity led to the formation of aggregates. Despite the decrease in Lac activity registered for the hybrid formulation, AgNPs-polymer-Lac, a significant augment in stability over time (up to 13days, at 50°C) was observed. This novel formulation displays improved synergistic performance over AgNPs-Lac or polymer-Lac conjugates, since in the former the Lac activity becomes residual at the end of 3days. By enabling many ionic interactions, chitosan restricted the mass transfer between Lac and substrate and, thus, inhibited the enzymatic activity. These hybrid nanocomposites made up of inorganic NPs, organic polymers and immobilized antimicrobial oxidoreductive enzymes represent a new class of materials with improved synergistic performance. Moreover, the Lac and the AgNPs different antimicrobial action, both in time and mechanism, may also constitute a new alternative to reduce the probability of developing resistance-associated mutations.	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.doi	10.1016/j.colsurfb.2017.03.023	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.6/7493
dc.language.iso	eng	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	pt_PT
dc.subject	Silver nanoparticles	pt_PT
dc.subject	Polymer stabilizer	pt_PT
dc.subject	Laccase	pt_PT
dc.subject	Synergistic effect	pt_PT
dc.subject	Enzymatic stability	pt_PT
dc.title	Synergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymers	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	220	pt_PT
oaire.citation.startPage	210	pt_PT
oaire.citation.title	Colloids and Surfaces B: Biointerfaces	pt_PT
oaire.citation.volume	154	pt_PT
person.familyName	Prado Felgueiras
person.familyName	Gouveia
person.familyName	Zille
person.givenName	Helena
person.givenName	Isabel Cristina
person.givenName	Andrea
person.identifier	912222
person.identifier	626131
person.identifier	456890
person.identifier.ciencia-id	8F17-D7C2-B4E9
person.identifier.ciencia-id	5711-86D0-A54B
person.identifier.ciencia-id	5217-8E4F-0C64
person.identifier.orcid	0000-0002-4354-0256
person.identifier.orcid	0000-0003-3290-9529
person.identifier.orcid	0000-0001-5299-4164
person.identifier.rid	H-4323-2017
person.identifier.rid	K-1651-2014
person.identifier.rid	B-3323-2008
person.identifier.scopus-author-id	55352304600
person.identifier.scopus-author-id	24328741500
person.identifier.scopus-author-id	7801341189
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT
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