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Effects of trans- and cis-resveratrol on Ca2+ handling in A7r5 vascular myocytes

2007Journal article Open access
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dc.contributor.authorVerde, Ignacio
dc.contributor.authorCampos-Toimil, Manuelpt
dc.contributor.authorElies, Jacobopt
dc.contributor.authorAlvarez, Ezequielpt
dc.contributor.authorOrallo, Franciscopt
dc.date.accessioned2010-04-28T10:07:28Z
dc.date.available2010-04-28T10:07:28Z
dc.date.issued2007
dc.description.abstractAlthough the natural polyphenol resveratrol posses a direct vasorelaxant effect, its effects on cytoplasmic Ca(2+) concentration ([Ca(2+)](i)) in vascular cells remain still unclear. Here, we have investigated the effects of the isomers trans- and cis-resveratrol on agonist- and high-K(+)-induced [Ca(2+)](i) increases and on voltage-activated transmembrane Ca(2+) fluxes using imaging and patch-clamp techniques in vascular A7r5 myocytes. Arginine vasopressin (AVP) or angiotensin II caused a biphasic increase in [Ca(2+)](i) that was reduced by preincubation with trans-resveratrol and cis-resveratrol. Both isomers also reduced the agonist-induced increase in [Ca(2+)](i) in absence of extracellular Ca(2+). In high-K(+) Ca(2+)-free solution, reintroduction of Ca(2+) caused a sustained rise in [Ca(2+)](i) that was reduced by preincubation with trans-resveratrol or cis-resveratrol. When the isomers were applied during the plateau phase of the agonist- or the high-K(+)-induced response, a biphasic change in [Ca(2+)](i) was observed: a transient reduction of the plateau (<5 min) followed by an increase (>10 min). Finally, trans-resveratrol and cis-resveratrol inhibited voltage-dependent L-type Ca(2+) currents (I(Ca(L))). In conclusion, resveratrol isomers exert a dual effect on [Ca(2+)](i) handling in A7r5 myocytes: 1) a blockade of I(Ca(L)) and 2) an increase in [Ca(2+)](i) by depletion of intracellular Ca(2+) stores (which interferes with the agonist-induced release of intracellular Ca(2+)) and influx of Ca(2+), mainly due to activation of capacitative Ca(2+) entry, although other Ca(2+)-permeable channels are also involved. Taken together, these effects may explain, in part, the endothelium-independent vasorelaxant effects of resveratrol in rat aorta.
dc.identifier.urihttp://hdl.handle.net/10400.6/617
dc.languageeng
dc.titleEffects of trans- and cis-resveratrol on Ca2+ handling in A7r5 vascular myocytespt
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue577:91-9pt
oaire.citation.titleEuropean Journal of Pharmacologypt
person.familyNameVerde
person.givenNameIgnacio
person.identifier.ciencia-idD012-1D7C-791A
person.identifier.orcid0000-0003-3492-5725
person.identifier.ridM-3991-2013
person.identifier.scopus-author-id55913729400
rcaap.rightsopenAccess
rcaap.typearticlept
relation.isAuthorOfPublicationbfb58284-b817-4267-9628-8fa150aa681c
relation.isAuthorOfPublication.latestForDiscoverybfb58284-b817-4267-9628-8fa150aa681c

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