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Bacteriocin production of the probiotic Lactobacillus acidophilus KS400

2018-09-27Journal article Open access
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dc.contributor.authorGaspar, C
dc.contributor.authorDonders, Gilbert
dc.contributor.authorOliveira, Rita Palmeira de
dc.contributor.authorQueiroz, João
dc.contributor.authorTomaz, C. T.
dc.contributor.authorOliveira, J. Martinez de
dc.contributor.authorOliveira, Ana Palmeira de
dc.date.accessioned2020-02-11T17:22:50Z
dc.date.available2020-02-11T17:22:50Z
dc.date.issued2018-09-27
dc.description.abstractIn the last years, the use of probiotics, including Lactobacillus species, has received much attention to prevent and treat vaginal disorders. These species have been described as having the ability to colonize the epithelial surface and produce antimicrobial metabolites that are able to control the remaining vaginal microflora. This study aimed to identify and characterize, for the first time, a bacteriocin natively produced by Lactobacillus acidophilus KS400 (probiotic strain from Gynoflor®-Medinova AG, Switzerland) and its antimicrobial activity against relevant urogenital pathogens. After organic acids and hydrogen peroxide neutralization in the fermented Lactobacillus acidophilus KS400 culture medium, bacteriocin activity was tested against the indicator microorganism Lactobacillus delbrueckii ATCC9649. The fermentation of Lactobacillus acidophilus KS400 for bacteriocin production was carried out in batch mode, and its antimicrobial activity, optical density and pH were monitored. After production and extraction, the bacteriocin molecular weight was estimated by electrophoresis and tested against vaginal pathogenic microorganisms. As described for other bacteriocins, batch fermentation profiles indicated that bacteriocin production occurs during the exponential growth phase of the lactobacilli, and declines during their stationary growth phase. The molecular weight of the bacteriocin is approximately 7.5 kDa. The bacteriocin containing protein extract was shown to inhibit the growth of Gardnerella vaginalis, Streptococcus agalactiae, Pseudomonas aeruginosa and the indicator strain Lactobacillus delbrueckii ATCC9649. We conclude that L. acidophilus KS400 produces bacteriocin with antimicrobial activity against relevant urogenital pathogens.pt_PT
dc.description.sponsorshipProject POCI-01-0145-FEDER-007491; Medinovapt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1186/s13568-018-0679-zpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/9220
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Openpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectLactobacillus acidophilus KS400pt_PT
dc.subjectVaginal probioticspt_PT
dc.subjectVaginal lactobacillipt_PT
dc.subjectBacteriocinspt_PT
dc.subjectVaginal infectionspt_PT
dc.titleBacteriocin production of the probiotic Lactobacillus acidophilus KS400pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00709%2F2013/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage153pt_PT
oaire.citation.titleAMB Expresspt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream5876
person.familyNameDonders
person.familyNamePalmeira-de-Oliveira
person.familyNameQueiroz
person.familyNameTomaz
person.familyNameMartinez Souto de Oliveira
person.familyNamePalmeira-de-Oliveira
person.givenNameGilbert
person.givenNameRita
person.givenNameJoão
person.givenNameCândida
person.givenNameJosé António
person.givenNameAna
person.identifier2198714
person.identifier.ciencia-id1213-39F2-65BB
person.identifier.ciencia-id931E-B66D-E341
person.identifier.ciencia-idFE13-0C44-13D1
person.identifier.ciencia-id8812-C62C-364F
person.identifier.ciencia-id7317-7078-C83A
person.identifier.orcid0000-0001-9890-6254
person.identifier.orcid0000-0001-9311-8355
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0002-0927-2331
person.identifier.orcid0000-0001-8608-0248
person.identifier.orcid0000-0003-0016-3769
person.identifier.ridL-3104-2014
person.identifier.ridF-9367-2014
person.identifier.ridE-5876-2013
person.identifier.scopus-author-id35734876900
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id7004546303
person.identifier.scopus-author-id35734956500
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationac20f769-1981-4cfc-9db2-41c30bba904f
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