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Resveratrol against Arcobacter butzleri and Arcobacter cryaerophilus: Activity and effect on cellular functions

2014Journal article Restricted access
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dc.contributor.authorFerreira, Susana
dc.contributor.authorSilva, Filomena
dc.contributor.authorQueiroz, João
dc.contributor.authorOleastro, Mónica
dc.contributor.authorDomingues, F.C.
dc.date.accessioned2020-01-29T14:36:30Z
dc.date.available2020-01-29T14:36:30Z
dc.date.issued2014
dc.description.abstractThe frequent isolation of Arcobacter butzleri and Arcobacter cryaerophilus from food samples makes it imperative to search for potential compounds able to inhibit the development of these bacteria. Taking this into consideration, this study focuses on the antimicrobial activity of resveratrol and its mechanism of action against A. butzleri and A. cryaerophilus. The activity of resveratrol was assessed by a microdilution method and time-kill curves. Resveratrol effect on cellular functions was assessed by flow cytometry evaluating intracellular DNA content and metabolic activity. Ethidium bromide (EtBr) accumulation in the presence of resveratrol was also evaluated, as well as the susceptibility to resveratrol in the presence of phenylalanine-arginine β-naphthylamide (PAβN). Scanning electron microscopy (SEM) was used to further evaluate cell damage caused by resveratrol. Resveratrol presented MIC values of 100 and 50μg/mL to A. butzleri and A. cryaerophilus, respectively. Based on the time-kill curves, resveratrol exhibited bactericidal activity, leading to a ≥3log10CFU/mL reduction of initial inoculums, for A. butzleri exponential phase cells incubated for 6h with 1× MIC or with 2× MIC after 24h for stationary phase cells. For A. cryaerophilus cells in exponential growth phase, 99.9% killing was achieved after 24h incubation with 2× MIC, whereas, for stationary phase cells, bactericidal activity was only detected after incubation with 4× MIC. Incubation with resveratrol led to a decrease in both intracellular DNA content and metabolic activity. An increase in the accumulation of EtBr was observed in the presence of resveratrol, and the efflux pump inhibitor PAβN reduced the MIC of resveratrol. SEM analysis revealed disintegration of A. butzleri cells treated with resveratrol, whereas no morphological alteration was observed for A. cryaerophilus cells. Resveratrol has a good anti-Arcobacter activity, and the results obtained suggest that this compound could act through several different mechanisms in the inhibition of this microorganism. The results encourage the use of this compound for the development of potential strategies to control Arcobacter in food products.pt_PT
dc.description.abstractSAU/UI0709/2011pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.ijfoodmicro.2014.04.004pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/8894
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationStrategic Project - UI 709 - 2011-2012
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0168160514001676?via%3Dihubpt_PT
dc.subjectArcobacterpt_PT
dc.subjectResveratrolpt_PT
dc.subjectAntimicrobial activitypt_PT
dc.subjectFlow cytometrypt_PT
dc.subjectEfflux pump activitypt_PT
dc.titleResveratrol against Arcobacter butzleri and Arcobacter cryaerophilus: Activity and effect on cellular functionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 709 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PTDC%2FAGR-ALI%2F121876%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FUI0709%2F2011/PT
oaire.citation.endPage68pt_PT
oaire.citation.startPage62pt_PT
oaire.citation.titleInternational Journal of Food Microbiologypt_PT
oaire.citation.volume180pt_PT
oaire.fundingStreamCOMPETE
oaire.fundingStream6820 - DCRRNI ID
person.familyNameFerreira
person.familyNameSilva
person.familyNameQueiroz
person.familyNameOleastro
person.familyNameDomingues
person.givenNameSusana
person.givenNameFilomena
person.givenNameJoão
person.givenNameMónica
person.givenNameFernanda
person.identifier1396147
person.identifier269435
person.identifier.ciencia-idF718-3569-E911
person.identifier.ciencia-idF91E-2365-F208
person.identifier.ciencia-id931E-B66D-E341
person.identifier.ciencia-id4115-04A9-FBCB
person.identifier.ciencia-id6514-0498-3E8F
person.identifier.orcid0000-0001-8308-2862
person.identifier.orcid0000-0003-2931-4583
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0001-6360-2576
person.identifier.orcid0000-0002-9540-0853
person.identifier.ridA-6810-2016
person.identifier.ridA-3091-2013
person.identifier.ridL-3104-2014
person.identifier.ridN-7295-2013
person.identifier.scopus-author-id34874668400
person.identifier.scopus-author-id35723100200
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id6603067224
person.identifier.scopus-author-id10139481400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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