Publication
Immobilization of bacteriophage in wound-dressing nanostructure
dc.contributor.author | Nogueira, Frederico | |
dc.contributor.author | Karumidze, Natia | |
dc.contributor.author | Kusradze, Ia | |
dc.contributor.author | Goderdzishvili, Marina | |
dc.contributor.author | Teixeira, Pilar | |
dc.contributor.author | Gouveia, Isabel C. | |
dc.date.accessioned | 2019-11-04T11:32:52Z | |
dc.date.available | 2019-11-04T11:32:52Z | |
dc.date.issued | 2017 | |
dc.description.abstract | Opportunistic bacteria that cause life-threatening infections are still a central problem associated with a healthcare setting. Bacteriophage capsid immobilization on nanostructured polymers maximizes its tail exposure and looks promising in applications toward skin-infections as alternative to antibiotics standardly used. The main goal of this work was to investigate the covalent immobilization of vB_Pae_Kakheti25 bacteriophage capsid on polycaprolactone (PCL) nanofibers (non-woven textile), as a potential effective antimicrobial, laundry resistant and non-toxic dressing for biomedical use. Surface analyses showed that the immobilization of vB_Pae_Kakheti25 bacteriophage capsid on PCL nanofibres oriented bacteriophage tails to interact with bacteria. Furthermore, antimicrobial assays showed a very effective 6 log bacterial reduction, which was equivalent to 99.9999%, after immediate and 2 hours of contact, even following 25 washing cycles (due to covalent bond). The activity of PCL-vB_Pae_Kakheti25 against P. aeruginosa was immediate and its reduction was complete. | pt_PT |
dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
dc.identifier.doi | 10.1016/j.nano.2017.08.008 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10400.6/7491 | |
dc.language.iso | eng | pt_PT |
dc.relation | COVALENT AND NON-COVALENT STRATEGIES FOR SURFACE MODIFICATION OF DIFFERENT TEXTILE MATERIALS WITH ANTIMICROBIAL PROPERTIES | |
dc.subject | Bacteriophages | pt_PT |
dc.subject | Antimicrobial agents | pt_PT |
dc.subject | Surface immobilization | pt_PT |
dc.subject | Electrospinning | pt_PT |
dc.subject | Pseudomonas aeruginosa | pt_PT |
dc.title | Immobilization of bacteriophage in wound-dressing nanostructure | pt_PT |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.awardTitle | COVALENT AND NON-COVALENT STRATEGIES FOR SURFACE MODIFICATION OF DIFFERENT TEXTILE MATERIALS WITH ANTIMICROBIAL PROPERTIES | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F91444%2F2012/PT | |
oaire.citation.endPage | 2484 | pt_PT |
oaire.citation.issue | 8 | pt_PT |
oaire.citation.startPage | 2475 | pt_PT |
oaire.citation.title | Nanomedicine: Nanotechnology, Biology and Medicine | pt_PT |
oaire.citation.volume | 13 | pt_PT |
person.familyName | Sequeira Nogueira | |
person.familyName | Karumidze | |
person.familyName | Araújo Teixeira | |
person.familyName | Gouveia | |
person.givenName | Frederico Álvaro | |
person.givenName | Natia | |
person.givenName | Maria do Pilar | |
person.givenName | Isabel Cristina | |
person.identifier | H-2141-2015 | |
person.identifier | 626131 | |
person.identifier.ciencia-id | 7119-F02C-91CE | |
person.identifier.ciencia-id | B01C-A9B6-9397 | |
person.identifier.ciencia-id | 5711-86D0-A54B | |
person.identifier.orcid | 0000-0003-2229-8920 | |
person.identifier.orcid | 0000-0002-3069-7123 | |
person.identifier.orcid | 0000-0003-2735-6608 | |
person.identifier.orcid | 0000-0003-3290-9529 | |
person.identifier.rid | K-1651-2014 | |
person.identifier.scopus-author-id | 56194664600 | |
person.identifier.scopus-author-id | 24328741500 | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
rcaap.rights | openAccess | pt_PT |
rcaap.type | article | pt_PT |
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