Repository logo
 
Publication

New insights for therapeutic recombinant human miRNAs heterologous production: Rhodovolum sulfidophilum vs Escherichia coli

2017Journal article Open access
Simple item page
dc.contributor.authorPereira, Patrícia
dc.contributor.authorPedro, Augusto
dc.contributor.authorQueiroz, João
dc.contributor.authorFigueiras, Ana R.
dc.contributor.authorSousa, Fani
dc.date.accessioned2020-01-09T15:49:03Z
dc.date.available2020-01-09T15:49:03Z
dc.date.issued2017
dc.description.abstractRNA interference-based technologies have emerged as an attractive and effective therapeutic option with potential application in diverse human diseases. These tools rely on the development of efficient strategies to obtain homogeneous non-coding RNA samples with adequate integrity and purity, thus avoiding non-targeted gene-silencing and related side-effects that impair their application onto pre-clinical practice. These RNAs have been preferentially obtained by in vitro transcription using DNA templates or via chemical synthesis. As an alternative to overcome the limitations presented by these methods, in vivo recombinant production of RNA biomolecules has become the focus in RNA synthesis research. Therefore, using pre-miR-29b as a model, here it is evaluated the time-course profile of Escherichia coli and Rhodovolum sulfidophilum microfactories to produce this microRNA. As the presence of major host contaminants arising from the biosynthesis process may have important implications in the subsequent downstream processing, it is also evaluated the production of genomic DNA and host total proteins. Considering the rapidly growing interest on these innovative biopharmaceuticals, novel, more cost-effective, simple and easily scaled-up technologies are highly desirable. As microRNA recombinant expression fulfills those requirements, it may take the leading edge in the methodologies currently available to obtain microRNAs for clinical or structural studies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPatrícia Pereira, Augusto Q. Pedro, João A. Queiroz, Ana R. Figueiras & Fani Sousa (2017) New insights for therapeutic recombinant human miRNAs heterologous production: Rhodovolum sulfidophilum vs Escherichia coli, Bioengineered, 8:5, 670-677, DOI: 10.1080/21655979.2017.1284710pt_PT
dc.identifier.doi10.1080/21655979.2017.1284710pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/8173
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.relation.publisherversionhttps://www.tandfonline.com/doi/full/10.1080/21655979.2017.1284710pt_PT
dc.subjectBioreactorspt_PT
dc.subjectEscherichia colipt_PT
dc.subjectGene Expression Regulationpt_PT
dc.subjectHumanspt_PT
dc.subjectMicroRNAspt_PT
dc.subjectRecombinationpt_PT
dc.subjectRhodovulumpt_PT
dc.subjectGeneticpt_PT
dc.subjectBacterialpt_PT
dc.titleNew insights for therapeutic recombinant human miRNAs heterologous production: Rhodovolum sulfidophilum vs Escherichia colipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/EXPL%2FBBB-BIO%2F1056%2F2012/PT
oaire.citation.endPage677pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage670pt_PT
oaire.citation.titleBioengineeredpt_PT
oaire.citation.volume8pt_PT
oaire.fundingStreamCOMPETE
person.familyNamePereira
person.familyNameQuaresma Henriques Pedro
person.familyNameQueiroz
person.familyNameFigueiras
person.familyNameSousa
person.givenNamePatrícia
person.givenNameAugusto
person.givenNameJoão
person.givenNameAna
person.givenNameFani
person.identifierhttps://scholar.google.com/citations?user=oer44UoAAAAJ&hl=pt-PT&oi=ao
person.identifier57602
person.identifier.ciencia-id4715-067B-4FD0
person.identifier.ciencia-id7111-B2BE-336D
person.identifier.ciencia-id931E-B66D-E341
person.identifier.ciencia-id6812-91D2-837E
person.identifier.ciencia-id991D-2E13-A840
person.identifier.orcid0000-0002-5665-5271
person.identifier.orcid0000-0001-8194-6022
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0001-8170-1113
person.identifier.orcid0000-0001-9996-2194
person.identifier.ridL-3104-2014
person.identifier.ridK-7332-2014
person.identifier.ridA-2014-2017
person.identifier.scopus-author-id56111844900
person.identifier.scopus-author-id54080426100
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id36771870800
person.identifier.scopus-author-id7005110268
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication157c4d6b-e0e0-4ef2-9d25-e461156ced0c
relation.isAuthorOfPublicatione105301c-3c7f-4705-961d-944d6db208dd
relation.isAuthorOfPublicationc798c033-c160-4f99-a8ad-04416923943f
relation.isAuthorOfPublication7189e119-c5cd-42cc-84fd-fca50b9d608c
relation.isAuthorOfPublication2935de97-fc90-4d38-9505-4caa364f8a10
relation.isAuthorOfPublication.latestForDiscovery2935de97-fc90-4d38-9505-4caa364f8a10
relation.isProjectOfPublication6cf70e84-77ab-4945-82a6-000932c5b7f6
relation.isProjectOfPublication.latestForDiscovery6cf70e84-77ab-4945-82a6-000932c5b7f6

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Pereira et al_Bioengineered_2017.pdf
Size:
1.25 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ICI - CICS | Documentos por Auto-Depósito
FCS - DCM | Documentos por Auto-Depósito