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Assessment of the Bioaccessibility and Bioavailability of the Phenolic Compounds of Prunus avium L. by in Vitro Digestion and Cell Model

2019Journal article Open access
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dc.contributor.authorGonçalves, Joana
dc.contributor.authorRamos, Rodrigo
dc.contributor.authorLuís, Ângelo
dc.contributor.authorRocha, Sandra
dc.contributor.authorRosado, Tiago
dc.contributor.authorGallardo, Eugenia
dc.contributor.authorDuarte, Ana Paula
dc.date.accessioned2020-01-28T15:53:00Z
dc.date.available2020-01-28T15:53:00Z
dc.date.issued2019
dc.description.abstractThe fruit of Prunus avium L., commonly known as sweet cherry, is an excellent source of phytochemicals, namely, phenolic compounds. This study aims to determine in vitro the bioavailability and the bioactivity of phenolic compounds present in cherries. Caco-2 cells were used, and the permeability and integrity of the monolayer formed were investigated. After incubation, the phenolic compounds that permeabilized the cell monolayer were quantified using a high-performance liquid chromatography–diode array detector, and the antioxidant activity was evaluated by the 1,1-diphenyl-2-picrylhydrazyl method. The results demonstrated that the phenolic compounds of sweet cherries after undergoing a simulated digestion were absorbed by the cellular barrier, becoming bioavailable. Contrary to what was found after incubation with the in vitro digested extract, the integrity of the cell monolayer was altered and its permeability increased upon incubation with the sweet cherry phenolic extract. Regarding the antioxidant activity, it was verified that this decreased after the absorption by the cellular monolayer. This study suggests that digestion is an indispensable process for absorption because without it the phenolic content of the food matrix does not become bioaccessible.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1021/acsomega.8b03499pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/8817
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.titleAssessment of the Bioaccessibility and Bioavailability of the Phenolic Compounds of Prunus avium L. by in Vitro Digestion and Cell Modelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00709%2F2013/PT
oaire.citation.endPage7613pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage7605pt_PT
oaire.citation.titleACS Omegapt_PT
oaire.citation.volume4pt_PT
oaire.fundingStream5876
person.familyNameGonçalves
person.familyNameSantos Luís
person.familyNameRocha
person.familyNameRosado
person.familyNameGallardo
person.familyNameDuarte
person.givenNameJoana
person.givenNameÂngelo Filipe
person.givenNameSandra
person.givenNameTiago
person.givenNameEugenia
person.givenNameAna Paula
person.identifier1918695
person.identifier2664004
person.identifier908303
person.identifier1255684
person.identifier.ciencia-id3D14-798B-65D3
person.identifier.ciencia-id7C1B-D6AB-9A28
person.identifier.ciencia-idC21B-DCC7-1C44
person.identifier.ciencia-idF617-5CA5-EF61
person.identifier.ciencia-idBC15-D376-80FE
person.identifier.orcid0000-0001-8702-415X
person.identifier.orcid0000-0003-0712-6522
person.identifier.orcid0000-0002-4686-2410
person.identifier.orcid0000-0003-0469-2895
person.identifier.orcid0000-0002-1802-8998
person.identifier.orcid0000-0003-3333-5977
person.identifier.ridE-3127-2015
person.identifier.ridM-7410-2017
person.identifier.ridB-5904-2009
person.identifier.scopus-author-id56307777400
person.identifier.scopus-author-id57191891820
person.identifier.scopus-author-id24721485300
person.identifier.scopus-author-id7102602131
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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