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Novel Methodology Based on Biomimetic Superhydrophobic Substrates to Immobilize Cells and Proteins in Hydrogel Spheres for Applications in Bone Regeneration

2013-02-14Journal article Restricted access
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dc.contributor.authorLima, Ana
dc.contributor.authorBatista, Patrícia Sofia Pinhanços
dc.contributor.authorValente, Tiago António Martins
dc.contributor.authorSilva, A. Sofia
dc.contributor.authorCorreia, Ilídio Joaquim Sobreira
dc.contributor.authorMano, João
dc.date.accessioned2018-03-20T09:30:13Z
dc.date.available2018-03-20T09:30:13Z
dc.date.issued2013-02-14
dc.description.abstractCell-based therapies for regenerative medicine have been characterized by the low retention and integration of injected cells into host structures. Cell immobilization in hydrogels for target cell delivery has been developed to circumvent this issue. In this work mesenchymal stem cells isolated from Wistar rats bone marrow (rMSCs) were immobilized in alginate beads fabricated using an innovative approach involving the gellification of the liquid precursor droplets onto biomimetic superhydrophobic surfaces without the need of any precipitation bath. The process occurred in mild conditions preventing the loss of cell viability. Furthermore, fibronectin (FN) was also immobilized inside alginate beads with high efficiency in order to mimic the composition of the extracellular matrix. This process occurred in a very fast way (around 5 min), at room temperature, without aggressive mechanical strengths or particle aggregation. The methodology employed allowed the production of alginate beads exhibiting a homogenous rMSCs and FN distribution. Encapsulated rMSCs remained viable and were released from the alginate for more than 20 days. In vivo assays were also performed, by implanting these particles in a calvarial bone defect to evaluate their potential for bone tissue regeneration. Microcomputed tomography and histological analysis results showed that this hybrid system accelerated bone regeneration process. The methodology employed had a dual role by preventing cell and FN loss and avoiding any contamination of the beads or exchange of molecules with the surrounding environment. In principle, the method used for cell encapsulation could be extended to other systems aimed to be used in tissue regeneration strategies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1089/ten.tea.2012.0249pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4642
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMary Ann Liebertpt_PT
dc.relationIsolation and Purification of Plasmid DNA for Cancer Therapy
dc.relationNEW HYDROGEL MICROSPHERES FROM NATURAL-ORIGIN POLYMERS FOR BIOTECHNOLOGICAL APLLICATIONS
dc.relationAEROSOLIZED GOLD-NANODEVICES FOR THERANOSTIC LUNG DELIVERY
dc.relationNOVA ABORDAGEM TERAPÊUTICA PARA A REGENERAÇÃO DA CARTILAGEM E DO OSSO
dc.relation.publisherversionhttps://www.liebertpub.com/doi/abs/10.1089/ten.tea.2012.0249pt_PT
dc.subjectBiomimeticpt_PT
dc.subjectHydrogelpt_PT
dc.subjectSuperhydrophobic Substratespt_PT
dc.subjectBone Regenerationpt_PT
dc.titleNovel Methodology Based on Biomimetic Superhydrophobic Substrates to Immobilize Cells and Proteins in Hydrogel Spheres for Applications in Bone Regenerationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleIsolation and Purification of Plasmid DNA for Cancer Therapy
oaire.awardTitleNEW HYDROGEL MICROSPHERES FROM NATURAL-ORIGIN POLYMERS FOR BIOTECHNOLOGICAL APLLICATIONS
oaire.awardTitleAEROSOLIZED GOLD-NANODEVICES FOR THERANOSTIC LUNG DELIVERY
oaire.awardTitleNOVA ABORDAGEM TERAPÊUTICA PARA A REGENERAÇÃO DA CARTILAGEM E DO OSSO
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEME-TME%2F103375%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FEBB-BIO%2F114320%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F71395%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F51584%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F45511%2F2008/PT
oaire.citation.endPage1187pt_PT
oaire.citation.startPage1175pt_PT
oaire.citation.titleTISSUE ENGINEERING: Part Apt_PT
oaire.citation.volume19pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876-PPCDTI
person.familyNameMatias da Silva
person.familyNameJoaquim Sobreira Correia
person.givenNameAna Sofia
person.givenNameIlídio
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-id7A1A-FEC8-7940
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0002-5388-1732
person.identifier.orcid0000-0003-1613-9675
person.identifier.scopus-author-id7003557499
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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