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Dual on–off and off–on switchable oligoaziridine biosensor

2013-01-15Journal article Restricted access
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dc.contributor.authorRaje, Vivek
dc.contributor.authorMorgado, Patrícia I.
dc.contributor.authorRibeiro, MP.
dc.contributor.authorCorreia, Ilídio Joaquim Sobreira
dc.contributor.authorBonifácio, Vasco
dc.contributor.authorBranco, Paula S.
dc.contributor.authorRicardo, Ana Aguiar
dc.date.accessioned2018-03-20T09:34:15Z
dc.date.available2018-03-20T09:34:15Z
dc.date.issued2013-01-15
dc.description.abstractA water-soluble biocompatible aziridine-based biosensor with pendant anthracene units was synthesized by radicalar polymerization of N-substituted aziridines in supercritical carbon dioxide. The binding ability of the sensor towards a series of metal ions was examined by comparing the fluorescence intensities of the solutions before and after the addition of 100 equivalents of a solution of the metal ion chloride salt. A fast, simple and highly optical sensitive dual behavior, “off–on” and “on–off” response, was observed after the biosensor was exposed to the metal cations in aqueous solution. Zinc presented the highest fluorescence enhancement (turn-on) and copper presented the highest fluorescence quenching (turn-off). The response time was found to be instantaneous and the detection limit was achieved even in the presence of excess metal cation competitors. By using immunofluorescence microscopy it was also shown that oligoaziridine acts as an “on–off” probe through highly sensitive (detection limit of 1.6 nM), selective and reversible binding to copper anions under physiologic conditions using living Human Fibroblast cells. The stoichiometry for the reaction of the biosensor with Cu2+ was determined by a Job plot and indicates the formation of an oligoaziridine-Cu2+ 1:2 adduct.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRaje, V.P., Morgado, P.I., Ribeiro, M.P., Correia, I.J., Bonifácio, V.D.B., Branco, P.S. e Aguiar-Ricardo, A. (2013) “Dual On-Off and Off-On Switchable Oligoaziridine Biosensor”, Biosensors and Bioelectronics, Vol. 39(1), pp. 64–69pt_PT
dc.identifier.doi10.1016/j.bios.2012.06.047pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4643
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationActivPOL - Development of oxazoline and aziridine-based antimicrobial polymers using supercritical carbon dioxide technology
dc.relationDEVELOPMENT OF HYDROGELS LOADED WITH MICROPARTICLES CONTAINING CELLS AND BIOMOLECULES FOR SKIN REGENERATION
dc.relationSYNTHESIS OF NEW MULTI-FUNCTIONAL POLYMERS WITH POTENTIAL THERAPEUTIC ACTION
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S095656631200406Xpt_PT
dc.subjectOligoaziridinespt_PT
dc.subjectBiosensorspt_PT
dc.subjectSupercritical carbon dioxidept_PT
dc.subjectCopperpt_PT
dc.subjectFluorescencept_PT
dc.titleDual on–off and off–on switchable oligoaziridine biosensorpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleActivPOL - Development of oxazoline and aziridine-based antimicrobial polymers using supercritical carbon dioxide technology
oaire.awardTitleDEVELOPMENT OF HYDROGELS LOADED WITH MICROPARTICLES CONTAINING CELLS AND BIOMOLECULES FOR SKIN REGENERATION
oaire.awardTitleSYNTHESIS OF NEW MULTI-FUNCTIONAL POLYMERS WITH POTENTIAL THERAPEUTIC ACTION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FQUI%2F73939%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEME-TME%2F103375%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/MIT-Pt%2FBS-CTRM%2F0051%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F80648%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/PIDDAC/SFRH%2FBPD%2F38014%2F2007/PT
oaire.citation.endPage69pt_PT
oaire.citation.startPage64pt_PT
oaire.citation.titleBiosensors and Bioelectronicspt_PT
oaire.citation.volume39pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamPIDDAC
person.familyNameDa Cruz Morgado Ferreira
person.familyNameRibeiro
person.familyNameJoaquim Sobreira Correia
person.familyNameBonifácio
person.familyNameBranco
person.familyNameAguiar-Ricardo
person.givenNamePatrícia Isabel
person.givenNameMaximiano
person.givenNameIlídio
person.givenNameVasco Daniel Bigas
person.givenNamePaula Cristina de Sério
person.givenNameAna
person.identifierUMbJ1KMAAAAJ
person.identifierbit.ly/google_scholar_bonifacio
person.identifier239073
person.identifier.ciencia-idE71C-3D0E-821B
person.identifier.ciencia-idBD11-6497-FD37
person.identifier.ciencia-idF610-7373-DC81
person.identifier.ciencia-idAE14-EB30-86A6
person.identifier.ciencia-id3D12-0B91-9DD0
person.identifier.ciencia-id2C1A-9947-19BA
person.identifier.orcid0000-0002-1005-9853
person.identifier.orcid0000-0001-6357-8115
person.identifier.orcid0000-0003-1613-9675
person.identifier.orcid0000-0003-2349-8473
person.identifier.orcid0000-0002-7312-8596
person.identifier.orcid0000-0002-2193-1440
person.identifier.ridC-3286-2011
person.identifier.scopus-author-id26658759200
person.identifier.scopus-author-id7003557499
person.identifier.scopus-author-id6602194100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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