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Molecular diagnosis of Arcobacter and Campylobacter in diarrhoeal samples among Portuguese patients

dc.contributor.authorFerreira, Susana
dc.contributor.authorJúlio, Cláudia
dc.contributor.authorQueiroz, João
dc.contributor.authorDomingues, F.C.
dc.contributor.authorOleastro, Mónica
dc.date.accessioned2020-01-29T14:39:07Z
dc.date.available2020-01-29T14:39:07Z
dc.date.issued2014
dc.description.abstractThe present study was conducted to investigate the prevalence and diversity of Arcobacter and Campylobacter spp. in 298 stool samples of patients with diarrhoea, collected from 22 Portuguese hospitals, between September and November 2012. Detection of Arcobacter and Campylobacter spp. was performed using molecular-based detection techniques, such as real-time fluorescence resonance energy transfer PCR, species-specific PCR, and sequencing of amplified PCR products. Overall, 1.3% of the samples were positive for Arcobacter butzleri and 0.3% for Arcobacter cryaerophilus. Campylobacter spp. were found in 31.9% of diarrhoeic faeces. Campylobacter jejuni and Campylobacter concisus were the most prevalent species (13.7% and 8.0%, respectively). The prevalence of Arcobacter and Campylobacter spp. was significantly different between children and adults (39.7% versus 22.8%, P = 0.003). We underline the high prevalence of these pathogens in diarrhoeal samples among Portuguese patients, with particular relevance in the paediatric age group.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.diagmicrobio.2013.11.021pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/8895
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationStrategic Project - UI 709 - 2011-2012
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0732889313006342pt_PT
dc.subjectArcobacterpt_PT
dc.subjectCampylobacterpt_PT
dc.subjectDiarrheapt_PT
dc.subjectGram-Negative Bacterial Infectionspt_PT
dc.subjectHumanspt_PT
dc.subjectPortugalpt_PT
dc.subjectMolecular detectionpt_PT
dc.titleMolecular diagnosis of Arcobacter and Campylobacter in diarrhoeal samples among Portuguese patientspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 709 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FUI0709%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F66857%2F2009/PT
oaire.citation.endPage225pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage220pt_PT
oaire.citation.titleDiagnostic Microbiology and Infectious Diseasept_PT
oaire.citation.volume78pt_PT
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStreamSFRH
person.familyNameFerreira
person.familyNameQueiroz
person.familyNameDomingues
person.familyNameOleastro
person.givenNameSusana
person.givenNameJoão
person.givenNameFernanda
person.givenNameMónica
person.identifier269435
person.identifier.ciencia-idF718-3569-E911
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person.identifier.orcid0000-0001-8308-2862
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0002-9540-0853
person.identifier.orcid0000-0001-6360-2576
person.identifier.ridA-6810-2016
person.identifier.ridL-3104-2014
person.identifier.ridN-7295-2013
person.identifier.scopus-author-id34874668400
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id10139481400
person.identifier.scopus-author-id6603067224
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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