Repository logo
 
Publication

Naphthalene amine support for G-quadruplex isolation

2017-08-07Journal article Restricted access
Simple item page
dc.contributor.authorFerreira, João
dc.contributor.authorSantos, Tiago
dc.contributor.authorPereira, Patrícia
dc.contributor.authorCorvo, Marta C.
dc.contributor.authorQueiroz, João
dc.contributor.authorSousa, Fani
dc.contributor.authorCruz, Carla
dc.date.accessioned2020-01-09T16:28:23Z
dc.date.available2020-01-09T16:28:23Z
dc.date.issued2017-08-07
dc.description.abstractG-quadruplex (G4) is involved in many biological processes, such as telomere function, gene expression and DNA replication. The selective isolation of G4 using affinity ligands that bind tightly and selectively is a valuable strategy for discovering new G4 binders for the separation of G4 from duplexes or the discrimination of G4 structures. In this work, one affinity chromatographic support was prepared using a naphthalene amine as a G4 binder. The ligand was immobilized on epoxy-activated Sepharose CL-6B using a long spacer arm and was characterized by HR-MAS spectroscopy. The supercoiled (sc) isoform of pVAX1-LacZ and pVAX1-G4 was isolated from a native sample. Also, the recovery and isolation of the plasmid isoforms from Escherichia coli lysate samples were achieved using an ionic gradient with different concentrations of NaCl in 10 mM Tris-HCl (pH 7.4). The retention times of different DNA/single strand sequences that can form G4, such as, c-MYC, c-kit1, c-kit2, tetrameric, telomeric (23AG), thrombin aptamer (TBA) and 58Sγ3 in this support were evaluated. Our experimental results suggest that the support exhibits selectivity for parallel c-MYC and c-kit1 G4s. In vitro transcription was performed using purified sc pVAX1-G4 and pPH600 to induce G4 formation and circular dichroism (CD) analysis confirmed that both transcripts adopt a parallel G4 topology.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1039/c7an00648apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/8177
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRoyal Society of Chemistrypt_PT
dc.relationNucleolin targeting by G-quadruplex aptamers for cervical cancer therapy
dc.relation.publisherversionhttps://pubs.rsc.org/en/content/articlelanding/2017/an/c7an00648a#!divAbstractpt_PT
dc.subjectCircular Dichroismpt_PT
dc.subjectEscherichia colipt_PT
dc.subjectPlasmidspt_PT
dc.subjectTelomerept_PT
dc.subjectAminespt_PT
dc.subjectG-Quadruplexespt_PT
dc.subjectNaphthalenespt_PT
dc.titleNaphthalene amine support for G-quadruplex isolationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNucleolin targeting by G-quadruplex aptamers for cervical cancer therapy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/EXPL%2FQEQ-MED%2F1068%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00959%2F2015%2FCP1300%2FCT0002/PT
oaire.citation.endPage2994pt_PT
oaire.citation.issue16pt_PT
oaire.citation.startPage2982pt_PT
oaire.citation.titleAnalystpt_PT
oaire.citation.volume142pt_PT
oaire.fundingStreamCOMPETE
oaire.fundingStreamInvestigador FCT
person.familyNameFerreira
person.familyNameAfonso dos Santos
person.familyNamePereira
person.familyNameCorvo
person.familyNameQueiroz
person.familyNameSousa
person.familyNameCruz
person.givenNameJoão
person.givenNameTiago André
person.givenNamePatrícia
person.givenNameMarta Cristina Parracho Cançado
person.givenNameJoão
person.givenNameFani
person.givenNameCarla
person.identifier.ciencia-id6517-B7D0-2B07
person.identifier.ciencia-id4715-067B-4FD0
person.identifier.ciencia-idBA1A-5C94-CA3A
person.identifier.ciencia-id931E-B66D-E341
person.identifier.ciencia-id991D-2E13-A840
person.identifier.ciencia-id6816-7888-F8B1
person.identifier.orcid0000-0002-3701-0515
person.identifier.orcid0000-0003-4620-0174
person.identifier.orcid0000-0002-5665-5271
person.identifier.orcid0000-0003-0890-6133
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0001-9996-2194
person.identifier.orcid0000-0001-6630-1242
person.identifier.ridL-3104-2014
person.identifier.ridA-2014-2017
person.identifier.ridI-7806-2013
person.identifier.scopus-author-id56111844900
person.identifier.scopus-author-id6602470713
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id7005110268
person.identifier.scopus-author-id7202701394
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2bbae65e-8d40-4f7c-8e75-3cfeb500c93a
relation.isAuthorOfPublicationb21d9803-f183-4785-b060-49c7859b4c17
relation.isAuthorOfPublication157c4d6b-e0e0-4ef2-9d25-e461156ced0c
relation.isAuthorOfPublication20ff6029-07a3-436d-8799-4d7206efd987
relation.isAuthorOfPublicationc798c033-c160-4f99-a8ad-04416923943f
relation.isAuthorOfPublication2935de97-fc90-4d38-9505-4caa364f8a10
relation.isAuthorOfPublication3fc51bf5-f849-4292-9aa3-4947fac1b9c8
relation.isAuthorOfPublication.latestForDiscoveryb21d9803-f183-4785-b060-49c7859b4c17
relation.isProjectOfPublication6b89537c-e46e-4cfd-8387-7fbeda4e72c5
relation.isProjectOfPublication0817baf0-da7c-4a7d-bf48-c5d6a0828e53
relation.isProjectOfPublication.latestForDiscovery6b89537c-e46e-4cfd-8387-7fbeda4e72c5

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Ferreira et al_Analyst_2017.pdf
Size:
4.71 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ICI - CICS | Documentos por Auto-Depósito
FCS - DCM | Documentos por Auto-Depósito