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Propolis Protects GC-1spg Spermatogonial Cells against Tert-Butyl Hydroperoxide-Induced Oxidative Damage

2024-01-03Journal article Open access
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dc.contributor.authorDuarte, Filipa Maia
dc.contributor.authorFeijó, Mariana
dc.contributor.authorLuís, Ângelo
dc.contributor.authorSocorro, Sílvia
dc.contributor.authorMaia, Cláudio J.
dc.contributor.authorCorreia, Sara
dc.date.accessioned2024-06-17T08:45:37Z
dc.date.available2024-06-17T08:45:37Z
dc.date.issued2024-01-03
dc.description.abstractPropolis is a natural resin produced by honeybees with plenty of pharmacologic properties, including antioxidant activity. Oxidative stress disrupts germ cell development and sperm function, with demonstrated harmful effects on male reproduction. Several natural antioxidants have been shown to reduce oxidative damage and increase sperm fertility potential; however, little is known about the effects of propolis. This work evaluated the role of propolis in protecting spermatogonial cells from oxidative damage. Propolis’ phytochemical composition and antioxidant potential were determined, and mouse GC-1spg spermatogonial cells were treated with 0.1–500 µg/mL propolis (12–48 h) in the presence or absence of an oxidant stimulus (tert-butyl hydroperoxide, TBHP, 0.005–3.6 µg/mL, 12 h). Cytotoxicity was assessed by MTT assays and proliferation by Ki-67 immunocytochemistry. Apoptosis, reactive oxygen species (ROS), and antioxidant defenses were evaluated colorimetrically. Propolis presented high phenolic and flavonoid content and moderate antioxidant activity, increasing the viability of GC-1spg cells and counteracting TBHP’s effects on viability and proliferation. Additionally, propolis reduced ROS levels in GC-1spg, regardless of the presence of TBHP. Propolis decreased caspase-3 and increased glutathione peroxidase activity in TBHP-treated GC-1spg cells. The present study shows the protective action of propolis against oxidative damage in spermatogonia, opening the possibility of exploiting its benefits to male fertility.pt_PT
dc.description.sponsorshipThe microscopy facility used in the development of this work is part of the PPBI-Portuguese Platform of BioImaging and is partially supported by Project POCI-01-0145-FEDER022122. The authors acknowledge Sandra Rebelo for kindly providing the GC-1spg cell line.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/ijms25010614pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/14432
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntioxidantpt_PT
dc.subjectOxidative stresspt_PT
dc.subjectPropolispt_PT
dc.subjectSpermatogonial cellspt_PT
dc.subjecttert-butyl hydroperoxidept_PT
dc.titlePropolis Protects GC-1spg Spermatogonial Cells against Tert-Butyl Hydroperoxide-Induced Oxidative Damagept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/2021.07367.BD/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage614pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume25pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
person.familyNamePombal Feijó
person.familyNameSantos Luís
person.familyNameSocorro
person.familyNameMaia Baptista
person.familyNameCorreia
person.givenNameMariana
person.givenNameÂngelo Filipe
person.givenNameSílvia Cristina da Cruz Marques
person.givenNameCláudio Jorge
person.givenNameSara
person.identifierhttps://scholar.google.pt/citations?user=PA3L_h8AAAAJ&hl=pt-PT
person.identifierhttps://scholar.google.pt/citations?user=hFKOd_QAAAAJ&hl=pt-PT&oi=ao
person.identifier.ciencia-idFC1F-F519-8104
person.identifier.ciencia-id3D14-798B-65D3
person.identifier.ciencia-idAB17-5C25-1F2C
person.identifier.ciencia-id5115-FCBD-A31F
person.identifier.ciencia-id7518-7FBC-A42F
person.identifier.orcid0000-0002-5330-5276
person.identifier.orcid0000-0003-0712-6522
person.identifier.orcid0000-0001-8181-488X
person.identifier.orcid0000-0002-5658-5445
person.identifier.orcid0000-0001-9199-8360
person.identifier.scopus-author-id23097994600
person.identifier.scopus-author-id23097426600
person.identifier.scopus-author-id7004157751
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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