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Determination of parathion in biological fluids by means of direct Solid Phase Microextraction.

2006Journal article Open access
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dc.contributor.authorGallardo, Eugenia
dc.contributor.authorBarroso, Mário
dc.contributor.authorMargalho, Cláudia
dc.contributor.authorCruz, Angelines
dc.contributor.authorVieira, Duarte Nuno
dc.contributor.authorLópez-Rivadulla, Manuelpt
dc.date.accessioned2010-04-28T10:07:29Z
dc.date.available2010-04-28T10:07:29Z
dc.date.issued2006
dc.description.abstractA new and simple procedure for the determination of parathion in human whole blood and urine using direct immersion (DI) solid-phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS) is presented. This technique was developed using only 100 ìL of sample, and ethion was used as internal standard (IS). A 65-ìm Carbowax/divinylbenzene (CW/DVB) SPME fibre was selected for sampling, and the main parameters affecting the SPME process such as extraction temperature, adsorption and desorption time, salt addition, agitation and pH effect were optimized to enhance the sensitivity of the method. This optimization was also performed to allow the qualitative determination of parathion’s main metabolite, paraoxon, in blood. The limits of detection and quantitation for parathion were 3 and 10 ng/mL for urine and 25 and 50 ng/mL for blood, respectively. For paraoxon, the limit of detection was 50 ng/mL in blood. The method showed linearity between the LOQ and 50 ìg/mL for both matrices, with correlation coefficients ranging from 0.9954 to 0.9999. Precision and accuracy were in conformity with the criteria normally accepted in bioanalytical method validation. The mean absolute recoveries were 35.1% for urine and 6.7% for blood. Other parameters such as dilution of sample and stability were also validated. Its simplicity and the fact that only 100 ìL of sample is required to accomplish the analysis make this method useful in forensic toxicology laboratories to determine this compound in intoxications, and it can be considered an alternative to other methods normally used for the determination of this compound in biological media.pt
dc.identifier.urihttp://hdl.handle.net/10400.6/622
dc.languageeng
dc.subjectDirect immersion solid-phase microextractionpt
dc.subjectParathion
dc.subjectWhole blood
dc.subjectUrine
dc.titleDetermination of parathion in biological fluids by means of direct Solid Phase Microextraction.pt
dc.typejournal article
dspace.entity.typePublication
person.familyNameGallardo
person.familyNameBarroso
person.familyNameMargalho
person.familyNameCRUZ LANDEIRA
person.familyNameVieira
person.givenNameEugenia
person.givenNameMário
person.givenNameCláudia
person.givenNameANGELINES
person.givenNameDuarte Nuno
person.identifier908303
person.identifier.ciencia-idF617-5CA5-EF61
person.identifier.ciencia-id5110-F476-ECCC
person.identifier.ciencia-idA211-521D-90CA
person.identifier.orcid0000-0002-1802-8998
person.identifier.orcid0000-0001-8848-2734
person.identifier.orcid0000-0003-1661-0367
person.identifier.orcid0000-0003-1382-4546
person.identifier.orcid0000-0002-7366-6765
person.identifier.ridM-7410-2017
person.identifier.ridKII-2916-2024
person.identifier.scopus-author-id24721485300
person.identifier.scopus-author-id35329243100
person.identifier.scopus-author-id8655072100
person.identifier.scopus-author-id7005983374
rcaap.rightsopenAccess
rcaap.typearticlept
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relation.isAuthorOfPublicationc1d97221-83ff-4292-9134-ce701c3adda7
relation.isAuthorOfPublication973723ae-b038-473d-b697-47f2b0b08038
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relation.isAuthorOfPublication.latestForDiscovery807238d9-0295-4103-b834-ae7fe6bd0e2d

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