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Characterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode Interface

2017-07-25Journal article Restricted access
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dc.contributor.authorNeto, Sónia de Fátima Estevão
dc.contributor.authorDiogo, Duarte Miguel de Melo
dc.contributor.authorCorreia, I.J.
dc.contributor.authorPaquete, Catarina
dc.contributor.authorLouro, Ricardo
dc.date.accessioned2018-03-22T09:21:54Z
dc.date.available2018-03-22T09:21:54Z
dc.date.issued2017-07-25
dc.description.abstractElectricity production in microbial fuel cells (MFCs) is an emerging green alternative to the use of fossil fuels. Shewanella oneidensis MR‐1 (SOMR‐1) is a Gram‐negative bacterium, adapted to MFCs due to its ability to link its bioenergetic metabolism through the periplasm to reduce extracellular electron acceptors. OmcA is a highly abundant outer‐membrane cytochrome of SOMR‐1 cells and is involved in the extracellular electron transfer to solid acceptors and electron shuttles. To investigate electron transfer performed by OmcA towards final acceptors, site directed mutagenesis was used to disturb the axial coordination of hemes. Interactions between OmcA and redox partners such as iron and graphene oxides, and electron shuttles were characterized using nuclear magnetic resonance and stopped‐flow experiments. Results showed that solid electron acceptors do not come into close proximity to the hemes, in agreement with experimentally observed slow electron transfer. In contrast, mutation of the distal axial ligand of heme VII changes the driving force of OmcA towards electron shuttles and reduces the affinity of the FMN:OmcA complex. Overall, these results reveal a functional specificity of particular hemes of OmcA and provide guidance for the rational design of mutated SOMR‐1 strains optimized for operating in different microbial electrochemical devices.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNeto, S. E., Melo‐Diogo, D., Correia, I. J., Paquete, C. M., & Louro, R. O. (2017). “Characterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode Interface.” Fuel Cells, Vol.17 (5), pp.601-611pt_PT
dc.identifier.doi10.1002/fuce.201700023pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4701
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationINVESTIGATING THE MOLECULAR DETAILS OF THE MULTI-ELECTRON REDUCTION OF NITROGEN COMPOUNDS BY OTR, A METALLOENZYME INVOLVED IN THE BIOGEOCHEMICAL CYCLE OF NITROGEN
dc.relationMULTIFUNCTIONAL GRAPHENE-OXIDE NANOCARRIERS FOR DRUG DELIVERY AND PHOTOTHERMAL THERAPY OF LUNG CANCER
dc.relationHealth Sciences Research Centre
dc.relationThe difference a cell wall makes: optimization of bioelectrochemical systems by exploring the paradigm of extracellular electron transfer in Gram-positive bacteria
dc.relationBioresources 4 Sustainability
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/abs/10.1002/fuce.201700023pt_PT
dc.subjectExtracellular Electron Transferpt_PT
dc.subjectFlavinspt_PT
dc.subjectGraphenept_PT
dc.subjectOuter membrane Cytochromespt_PT
dc.subjectShewanella oneidensis MR-1pt_PT
dc.titleCharacterization of OmcA Mutants from Shewanella oneidensis MR‐1 to Investigate the Molecular Mechanisms Underpinning Electron Transfer Across the Microbe‐Electrode Interfacept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleINVESTIGATING THE MOLECULAR DETAILS OF THE MULTI-ELECTRON REDUCTION OF NITROGEN COMPOUNDS BY OTR, A METALLOENZYME INVOLVED IN THE BIOGEOCHEMICAL CYCLE OF NITROGEN
oaire.awardTitleMULTIFUNCTIONAL GRAPHENE-OXIDE NANOCARRIERS FOR DRUG DELIVERY AND PHOTOTHERMAL THERAPY OF LUNG CANCER
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleThe difference a cell wall makes: optimization of bioelectrochemical systems by exploring the paradigm of extracellular electron transfer in Gram-positive bacteria
oaire.awardTitleBioresources 4 Sustainability
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F96952%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F103506%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F00709%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBBB-BQB%2F4178%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04551%2F2013/PT
oaire.citation.endPage611pt_PT
oaire.citation.startPage601pt_PT
oaire.citation.titleFuel Cellspt_PT
oaire.citation.volume17pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMiguel de Melo Diogo
person.familyNameJoaquim Sobreira Correia
person.familyNamePaquete
person.familyNameLouro
person.givenNameDuarte
person.givenNameIlídio
person.givenNameCatarina
person.givenNameRicardo
person.identifierC2z5x04AAAAJ
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-id9C10-9BD8-634E
person.identifier.ciencia-idF610-7373-DC81
person.identifier.ciencia-idA01E-576D-43B7
person.identifier.orcid0000-0001-9984-3603
person.identifier.orcid0000-0003-1613-9675
person.identifier.orcid0000-0002-1511-3732
person.identifier.orcid0000-0002-2392-6450
person.identifier.ridA-6615-2016
person.identifier.scopus-author-id56266511300
person.identifier.scopus-author-id7003557499
person.identifier.scopus-author-id6507206247
person.identifier.scopus-author-id6603724134
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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