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Biofunctionalized nanoparticles with pH-responsive and cell penetrating blocks for gene delivery

2013-06-13Journal article Restricted access
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dc.contributor.authorGaspar, Vítor Manuel Abreu
dc.contributor.authorMarques, João Filipe Gonçalves
dc.contributor.authorSousa, Fani
dc.contributor.authorLouro, Ricardo
dc.contributor.authorQueiroz, João
dc.contributor.authorCorreia, I.J.
dc.date.accessioned2018-03-20T09:27:51Z
dc.date.available2018-03-20T09:27:51Z
dc.date.issued2013-06-13
dc.description.abstractBridging the gap between nanoparticulate delivery systems and translational gene therapy is a long sought after requirement in nanomedicine-based applications. However, recent developments regarding nanoparticle functionalization have brought forward the ability to synthesize materials with biofunctional moieties that mimic the evolved features of viral particles. Herein we report the versatile conjugation of both cell penetrating arginine and pH-responsive histidine moieties into the chitosan polymeric backbone, to improve the physicochemical characteristics of the native material. Amino acid coupling was confirmed by 2D TOCSY NMR and Fourier transform infrared spectroscopy. The synthesized chitosan–histidine–arginine (CH–H–R) polymer complexed plasmid DNA biopharmaceuticals, and spontaneously assembled into stable 105 nm nanoparticles with spherical morphology and positive surface charge. The functionalized delivery systems were efficiently internalized into the intracellular compartment, and exhibited remarkably higher transfection efficiency than unmodified chitosan without causing any cytotoxic effect. Additional findings regarding intracellular trafficking events reveal their preferential escape from degradative lysosomal pathways and nuclear localization. Overall, this assembly of nanocarriers with bioinspired moieties provides the foundations for the design of efficient and customizable materials for cancer gene therapy.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGaspar, V.M, Marques, J.G, Sousa, F, Louro, R.O, Queiroz, J.A e Correia, I.J. (2013) "Biofunctionalized Nanoparticles with pH-responsive and Cell Penetrating Blocks for Gene Delivery", Nanotechnology, Vol. 24(27), nº do artigo 275101pt_PT
dc.identifier.doi10.1088/0957-4484/24/27/275101pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4641
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherIOP Publishingpt_PT
dc.relationIsolation and Purification of Plasmid DNA for Cancer Therapy
dc.relationStrategic Project - UI 709 - 2011-2012
dc.relationBIOSYNTHESIS AND PURIFICATION OF MINICIRCLE DNA FOR APPLICATION IN DIABETES CELL-SPECIFIC GENE THERAPY
dc.relation.publisherversionhttp://iopscience.iop.org/article/10.1088/0957-4484/24/27/275101/metapt_PT
dc.subjectGene deliverypt_PT
dc.subjectNanoparticlespt_PT
dc.titleBiofunctionalized nanoparticles with pH-responsive and cell penetrating blocks for gene deliverypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleIsolation and Purification of Plasmid DNA for Cancer Therapy
oaire.awardTitleStrategic Project - UI 709 - 2011-2012
oaire.awardTitleBIOSYNTHESIS AND PURIFICATION OF MINICIRCLE DNA FOR APPLICATION IN DIABETES CELL-SPECIFIC GENE THERAPY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEME-TME%2F103375%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FEBB-BIO%2F114320%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FUI0709%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F80402%2F2011/PT
oaire.citation.startPage275101pt_PT
oaire.citation.titleNanotechnologypt_PT
oaire.citation.volume24pt_PT
oaire.fundingStream3599-PPCDT
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oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStreamFARH
person.familyNameGaspar
person.familyNameSousa
person.familyNameLouro
person.familyNameQueiroz
person.familyNameJoaquim Sobreira Correia
person.givenNameVítor
person.givenNameFani
person.givenNameRicardo
person.givenNameJoão
person.givenNameIlídio
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-id6F16-3640-73E3
person.identifier.ciencia-id991D-2E13-A840
person.identifier.ciencia-id931E-B66D-E341
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0002-0372-2493
person.identifier.orcid0000-0001-9996-2194
person.identifier.orcid0000-0002-2392-6450
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0003-1613-9675
person.identifier.ridB-1602-2017
person.identifier.ridA-2014-2017
person.identifier.ridL-3104-2014
person.identifier.scopus-author-id36968590900
person.identifier.scopus-author-id7005110268
person.identifier.scopus-author-id6603724134
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id7003557499
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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