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Separation of different forms of proteose peptone 3 by hydrophobic interaction chromatography with a dual salt system

2008-01-18Journal article Restricted access
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dc.contributor.authorSousa, Ângela Maria Almeida de
dc.contributor.authorPassarinha, Luís António Paulino
dc.contributor.authorRodrigues, L.R.
dc.contributor.authorTeixeira, José
dc.contributor.authorMendonça, António
dc.contributor.authorQueiroz, João
dc.date.accessioned2022-08-24T10:20:24Z
dc.date.available2022-08-24T10:20:24Z
dc.date.issued2008-01-18
dc.description.abstractA panel of four hydrophobic adsorbents (butyl-, octyl-, phenyl- and epoxy-Sepharose) was used to examine the selectivity and fractionation of several proteose peptone 3 (PP3) forms from a freeze-dried extract of whey bovine milk. In particular, the effects of altering the ligand type and salt were investigated. The chromatographic studies suggest that PP3 strongly interacts among the three commercial hydrophobic resins leading to a drop off in selectivity, while a complete binding was achieved at low salt concentrations (below 0.5 M) and total elution only with phosphate buffer and/or water stepwise conditions. Only in epoxy–Sepharose was an appreciably selectivity of the several fractions of PP3 present in the initial feedstock attained. Despite the high salt concentration for a complete binding of PP3 (above 1.5 M ammonium sulfate) onto this support, the dual salt system (ammonium sulfate 1 M and sodium citrate 0.8 M) led to a high separation degree of high and low molecular weight forms of PP3.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSousa, A., Passarinha, L. A., Rodrigues, L. R., Teixeira, J. A., Mendonça, A., & Queiroz, J. A. (2008). Separation of different forms of proteose peptone 3 by hydrophobic interaction chromatography with a dual salt system. Biomedical Chromatography, 22(5), 447-449. doi:10.1002/bmc.961pt_PT
dc.identifier.doi10.1002/bmc.961pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/12313
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJohn Wiley & Sons, Ltdpt_PT
dc.relation.publisherversionhttps://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/bmc.961pt_PT
dc.subjectProteose peptone 3pt_PT
dc.subjectHydrophobic adsorbentspt_PT
dc.subjectDual salt systempt_PT
dc.titleSeparation of different forms of proteose peptone 3 by hydrophobic interaction chromatography with a dual salt systempt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage449pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage447pt_PT
oaire.citation.titleBiomedical Chromatographypt_PT
oaire.citation.volume22pt_PT
person.familyNameSousa
person.familyNamePaulino Passarinha
person.familyNameMARONA RODRIGUES
person.familyNameTeixeira
person.familyNameMendonça
person.familyNameQueiroz
person.givenNameÂngela
person.givenNameLuís António
person.givenNameLIGIA RAQUEL
person.givenNameJosé
person.givenNameAntónio
person.givenNameJoão
person.identifier817754
person.identifierhttps://www.researchgate.net/profile/Luis_Passarinha
person.identifierC-3151-2009
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person.identifier.ciencia-id2419-6E20-F958
person.identifier.ciencia-id931E-B66D-E341
person.identifier.orcid0000-0001-9155-7581
person.identifier.orcid0000-0001-6910-7576
person.identifier.orcid0000-0001-9265-0630
person.identifier.orcid0000-0002-4918-3704
person.identifier.orcid0000-0001-8069-0440
person.identifier.orcid0000-0002-3096-8325
person.identifier.ridA-6268-2012
person.identifier.ridI-3093-2012
person.identifier.ridL-3104-2014
person.identifier.scopus-author-id24330268800
person.identifier.scopus-author-id12445439600
person.identifier.scopus-author-id55938875200
person.identifier.scopus-author-id13402823200
person.identifier.scopus-author-id56890866600
person.identifier.scopus-author-id7003705645
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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