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Proteome analysis of vitreous humor in retinal detachment using two different flow-charts for protein fractionation

2017Journal article Open access
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dc.contributor.authorGaspar, Leonor Isabel Mesquita
dc.contributor.authorSantos, F.M.
dc.contributor.authorAlbuquerque, Tânia
dc.contributor.authorSousa, João P. Castro de
dc.contributor.authorPassarinha, L A
dc.contributor.authorTomaz, Cândida T.
dc.date.accessioned2020-02-07T15:48:05Z
dc.date.available2022-07-29T00:30:18Z
dc.date.issued2017
dc.description.abstractThe deeper understanding of retinal detachment (RD) pathogenesis may improve the visual outcome after surgery. Given the main role of the vitreous in retinal eye diseases, two strategies were explored to identify its proteome in RD. Fractionation techniques such as anion exchange chromatography (IEX) and SDS-PAGE combined with MALDI-TOF/TOF analysis allowed to identify 127 proteins in vitreous of RD patients. From these proteins, 19 were identified using only the IEX fractionation strategy, and 117 using a bidimensional (IEX and SDS-PAGE) fractionation. Of these proteins, 68 had not yet been found in other vitreous proteomic studies. The fractionation with IEX and SDS-PAGE largely improved the number of identified proteins proving that it is crucial to combine several methodologies to cover vitreous proteome.pt_PT
dc.description.sponsorshipCENTRO-07-ST24-FEDER-002014;Novartis Farma-Produtos Farmacêuticos, SA; Project POCI-01-0145-FEDER-007491pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jchromb.2017.07.049pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/9143
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationVitreous humor biomarkers in Age-related Macular Degeneration
dc.subjectProteomic Analysispt_PT
dc.subjectMass spectrometrypt_PT
dc.subjectRetinal Detachmentpt_PT
dc.subjectVitreous Humourpt_PT
dc.subjectElectrophoresispt_PT
dc.subjectLIquid Chromatographypt_PT
dc.titleProteome analysis of vitreous humor in retinal detachment using two different flow-charts for protein fractionationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleVitreous humor biomarkers in Age-related Macular Degeneration
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F112526%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00709%2F2013/PT
oaire.citation.endPage341pt_PT
oaire.citation.startPage334pt_PT
oaire.citation.titleJournal of Chromatography Bpt_PT
oaire.citation.volume1061-1062pt_PT
oaire.fundingStreamPOR_CENTRO
oaire.fundingStream5876
person.familyNameGaspar
person.familyNameMilhano dos Santos
person.familyNamePaulino Passarinha
person.familyNameTomaz
person.givenNameLeonor Isabel Mesquita
person.givenNameFátima Raquel
person.givenNameLuís António
person.givenNameCândida
person.identifierhttps://www.researchgate.net/profile/Luis_Passarinha
person.identifier2198714
person.identifier.ciencia-id3216-14E9-65E0
person.identifier.ciencia-idD01A-2666-0E20
person.identifier.ciencia-id621B-74BF-F9A7
person.identifier.ciencia-idFE13-0C44-13D1
person.identifier.orcid0000-0002-5106-1888
person.identifier.orcid0000-0002-4041-1504
person.identifier.orcid0000-0001-6910-7576
person.identifier.orcid0000-0002-0927-2331
person.identifier.ridF-9367-2014
person.identifier.scopus-author-id12445439600
person.identifier.scopus-author-id7004546303
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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