Repository logo
 
Publication

A new strategy for RNA isolation from eukaryotic cells using arginine affinity chromatography

2012-06-02Journal article Open access
Simple item page
dc.contributor.authorMartins, Ana Rita Nunes
dc.contributor.authorMaia, C J
dc.contributor.authorQueiroz, João
dc.contributor.authorSousa, Fani
dc.date.accessioned2019-11-27T17:13:24Z
dc.date.available2019-11-27T17:13:24Z
dc.date.issued2012-06-02
dc.description.abstractThe relevance of RNA in many biological functions has been recognized, broadening the scope of RNA research activities, from basic to applied sciences, also aiming the translation to clinical fields. The preparation and purification of RNA is a critical step for further application, since the quality of the template is crucial to ensure reproducibility and biological relevance. Therefore, the establishment of new tools that allows the isolation of pure RNA with high quality is of particular importance. New chromatographic strategies for RNA purification were considered, exploiting affinity interactions between amino acids and nucleic acids. In the present study, a single arginine-affinity chromatography step was employed for the purification of RNA from a total eukaryotic nucleic acid extract, thus eliminating several steps compared with current RNA isolation procedures. The application of this process resulted in a high RNA recovery yield of 96 ± 17% and the quality control analysis revealed a high integrity (28S:18S ratio = 1.96) in RNA preparations as well as a good purity, demonstrated by the scarce detection of proteins and the reduction on genomic DNA contamination to residual concentrations. Furthermore, the performance of the new RNA isolation method was tested regarding the applicability of the isolated RNA in modern molecular biology techniques. Hence, this new affinity approach will simplify the isolation and purification of RNA, which can bring great improvements in biomedical investigation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/jssc.201200338pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/7634
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationIsolation and Purification of Plasmid DNA for Cancer Therapy
dc.relationBIORECONHECIMENTO POR CROMATOGRAFIA DE AFINIDADE COM AMINOÁCIDOS NA PURIFICAÇÃO DE RNA
dc.subjectAffinity chromatographypt_PT
dc.subjectArgininept_PT
dc.subjectGene expressionpt_PT
dc.subjectReal-time PCRpt_PT
dc.subjectRNA isolationpt_PT
dc.titleA new strategy for RNA isolation from eukaryotic cells using arginine affinity chromatographypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleIsolation and Purification of Plasmid DNA for Cancer Therapy
oaire.awardTitleBIORECONHECIMENTO POR CROMATOGRAFIA DE AFINIDADE COM AMINOÁCIDOS NA PURIFICAÇÃO DE RNA
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FEBB-BIO%2F114320%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F64100%2F2009/PT
oaire.citation.endPage3226pt_PT
oaire.citation.issue22pt_PT
oaire.citation.startPage3217pt_PT
oaire.citation.titleJournal of Separation Sciencept_PT
oaire.citation.volume35pt_PT
oaire.fundingStream5876-PPCDTI
oaire.fundingStreamFARH
person.familyNameNunes Martins
person.familyNameMaia Baptista
person.familyNameQueiroz
person.familyNameSousa
person.givenNameAna Rita
person.givenNameCláudio Jorge
person.givenNameJoão
person.givenNameFani
person.identifierhttps://scholar.google.pt/citations?user=hFKOd_QAAAAJ&hl=pt-PT&oi=ao
person.identifier.ciencia-id2E16-5E6C-7EFD
person.identifier.ciencia-id5115-FCBD-A31F
person.identifier.ciencia-id931E-B66D-E341
person.identifier.ciencia-id991D-2E13-A840
person.identifier.orcid0000-0002-2930-7028
person.identifier.orcid0000-0002-5658-5445
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0001-9996-2194
person.identifier.ridL-3104-2014
person.identifier.ridA-2014-2017
person.identifier.scopus-author-id23097426600
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id7005110268
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa2ca0e85-2923-4684-b856-2d5ef2590b21
relation.isAuthorOfPublication1080c532-c737-4b60-bc44-1d18e3c877d7
relation.isAuthorOfPublicationc798c033-c160-4f99-a8ad-04416923943f
relation.isAuthorOfPublication2935de97-fc90-4d38-9505-4caa364f8a10
relation.isAuthorOfPublication.latestForDiscoverya2ca0e85-2923-4684-b856-2d5ef2590b21
relation.isProjectOfPublication356d89ac-2690-4f65-b2ef-c2fec8cbfa34
relation.isProjectOfPublicationea963287-c0bb-4120-a337-1d26d00ef98e
relation.isProjectOfPublication.latestForDiscovery356d89ac-2690-4f65-b2ef-c2fec8cbfa34

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
A new strategy for RNA isolation fromeukaryotic cells using arginine affinitychromatography.pdf
Size:
685.68 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

FCS - DCM | Documentos por Auto-Depósito
ICI - CICS | Documentos por Auto-Depósito