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Antimicrobial, antibiofilm and cytotoxic activities of Hakea sericea Schrader extracts

2014Journal article Restricted access
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dc.contributor.authorLuís, Ângelo
dc.contributor.authorBreintenfeld, Luiza
dc.contributor.authorFerreira, Susana
dc.contributor.authorDuarte, Ana Paula
dc.contributor.authorDomingues, F.C.
dc.date.accessioned2020-01-29T14:33:07Z
dc.date.available2020-01-29T14:33:07Z
dc.date.issued2014
dc.description.abstractBackground: Hakea sericea Schrader is an invasive shrub in Portuguese forests. Objective: The goal of this work was to evaluate the antimicrobial activity of H. sericea extracts against several strains of microorganisms, including the ability to inhibit the formation of biofilms. Additionally the cytotoxic properties of these extracts, against human cells, were assessed. Materials and Methods: The antimicrobial activity of the methanolic extracts of H. sericea was assessed by disk diffusion assay and Minimum Inhibitory Concentration (MIC) value determination. The antibiofilm activity was determined by quantification of total biofilm biomass with crystal violet. Cytotoxicity was evaluated by hemolysis assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. Results: For Gram-positive bacteria, MIC values of H. sericea methanolic extracts ranged between 0.040 and 0.625 mg/mL, whereas the fruits extract yielded the lowest MIC for several strains of microorganisms, namely, S. aureus, B. cereus, L. monocytogenes and clinical methicillin-resistant S. aureus (MRSA). Stems and fruits extract at 2.5 mg/mL effectively eradicated the biofilm of S. aureus ATCC 25923, SA 01/10 and MRSA 12/10. Regarding leaves extract, hemolysis was not observed, and in the case of stems and fruits, hemolysis was verified only for higher concentrations, suggesting its low toxicity. Fruits extract presented no toxic effect to normal human dermal fibroblasts (NHDF) cells however for concentrations of 0.017 and 0.008 mg/mL this extract was able to decrease human breast adenocarcinoma cells (MCF-7) viability in about 60%, as MTT test results had confirmed. This is a clearly demonstrator of the cytotoxicity of this extract against MCF-7 cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.4103/0973-1296.127331pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/8893
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationStrategic Project - UI 709 - 2011-2012
dc.relationPESQUISA E IDENTIFICAÇAO DE COMPOSTOS BIOACTIVOS EM PLANTAS FLORESTAIS
dc.relation.publisherversionhttp://www.phcog.com/article.asp?issn=0973-1296;year=2014;volume=10;issue=37;spage=6;epage=13;aulast=Lu%EDspt_PT
dc.subjectAntibiofilmpt_PT
dc.subjectAntimicrobialpt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectHakea sericeapt_PT
dc.titleAntimicrobial, antibiofilm and cytotoxic activities of Hakea sericea Schrader extractspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStrategic Project - UI 709 - 2011-2012
oaire.awardTitlePESQUISA E IDENTIFICAÇAO DE COMPOSTOS BIOACTIVOS EM PLANTAS FLORESTAIS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FUI0709%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F65238%2F2009/PT
oaire.citation.issue37pt_PT
oaire.citation.startPage6pt_PT
oaire.citation.titlePharmacognosy Magazinept_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6820 - DCRRNI ID
person.familyNameSantos Luís
person.familyNameGranadeiro
person.familyNameFerreira
person.familyNameDuarte
person.familyNameDomingues
person.givenNameÂngelo Filipe
person.givenNameLuiza Breitenfeld
person.givenNameSusana
person.givenNameAna Paula
person.givenNameFernanda
person.identifier274116
person.identifier1255684
person.identifier.ciencia-id3D14-798B-65D3
person.identifier.ciencia-idE71F-B2E5-D657
person.identifier.ciencia-idF718-3569-E911
person.identifier.ciencia-idBC15-D376-80FE
person.identifier.ciencia-id6514-0498-3E8F
person.identifier.orcid0000-0003-0712-6522
person.identifier.orcid0000-0001-8550-1645
person.identifier.orcid0000-0001-8308-2862
person.identifier.orcid0000-0003-3333-5977
person.identifier.orcid0000-0002-9540-0853
person.identifier.ridP-1852-2015
person.identifier.ridA-6810-2016
person.identifier.ridB-5904-2009
person.identifier.ridN-7295-2013
person.identifier.scopus-author-id34467456600
person.identifier.scopus-author-id34874668400
person.identifier.scopus-author-id7102602131
person.identifier.scopus-author-id10139481400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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