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Anti-Candida activity of a chitosan hydrogel: mechanism of action and cytotoxicity profile

2010-10-16Journal article Restricted access
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dc.contributor.authorOliveira, Ana Palmeira de
dc.contributor.authorRibeiro, MP.
dc.contributor.authorOliveira, Rita Manuela Palmeira de
dc.contributor.authorGaspar, Carlos
dc.contributor.authorOliveira, Sofia Costa de
dc.contributor.authorCorreia, I.J.
dc.contributor.authorVaz, Cidália Pina
dc.contributor.authorOliveira, José António Martinez Souto de
dc.contributor.authorQueiroz, João
dc.contributor.authorRodrigues, Acácio
dc.date.accessioned2018-03-16T14:17:16Z
dc.date.available2018-03-16T14:17:16Z
dc.date.issued2010-10-16
dc.description.abstractCandida spp. are common causative agents of mucocutaneous infections. New therapeutic antifungal drugs are needed to treat chronic disease as these are frequently clinically resistant to azols. Chitosan, among other possible vehicles for active compounds, shows an added value as it appears to have intrinsic antimicrobial properties. The aim of the present study was to evaluate the anti-Candida activity of a medium-molecular-weight chitosan hydrogel (CH), to clarify its possible mechanism of action and to evaluate its cytotoxicity on human fibroblasts. CH antifungal activity was assessed according to CLSI reference M27-A3 protocol; its mechanism of action was investigated by flow cytometry, and its cytotoxicity was studied by MTT assay. CH demonstrated a full inhibition of C. tropicalis, C. krusei, C. guilliermondii and C. parapsilosis growth while impairing C. albicans and C. glabrata viability. Flow cytometry tests showed that CH acts by inducing primary lesion of the cytoplasmic membrane. However, CH showed no cytotoxic effect upon human fibroblasts cells. Resistant strains will require new therapeutic approaches. Chitosan being a good carrier and having itself anti-Candida activity seems to be a promising vehicle to be used for the treatment of mucocutaneous candidosis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPalmeira-de-Oliveira, A., Ribeiro, M.P., Palmeira-de-Oliveira, R., Gaspar, C., Costa-de-Oliveira, S., Correia, I.J., Queiroz, J.A., Pina Vaz C., Martinez-de-Oliveira, J. e Rodrigues, A.G. (2010) “Anticandida Activity of a Chitosan Hydrogel: Mechanism of Action and Cytotoxicity Profile”, Gynecologic and Obstetric Investigation Journal, Vol. 70(4), pp. 322-327pt_PT
dc.identifier.doi10.1159/000314023pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4625
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherKARGERpt_PT
dc.relation.publisherversionhttps://www.karger.com/Article/Abstract/314023pt_PT
dc.subjectChitosan hydrogelpt_PT
dc.subjectAnticandida activitypt_PT
dc.subjectBiodegradablept_PT
dc.titleAnti-Candida activity of a chitosan hydrogel: mechanism of action and cytotoxicity profilept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage327pt_PT
oaire.citation.startPage322pt_PT
oaire.citation.titleGynecologic and Obstetric Investigationpt_PT
oaire.citation.volume70pt_PT
person.familyNamePalmeira-de-Oliveira
person.familyNameRibeiro
person.familyNameJoaquim Sobreira Correia
person.familyNamePina-Vaz
person.familyNameMartinez Souto de Oliveira
person.familyNameQueiroz
person.givenNameAna
person.givenNameMaximiano
person.givenNameIlídio
person.givenNameCidália
person.givenNameJosé António
person.givenNameJoão
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-id7317-7078-C83A
person.identifier.ciencia-idBD11-6497-FD37
person.identifier.ciencia-idF610-7373-DC81
person.identifier.ciencia-id6B1F-2A7A-A967
person.identifier.ciencia-id8812-C62C-364F
person.identifier.ciencia-id931E-B66D-E341
person.identifier.orcid0000-0003-0016-3769
person.identifier.orcid0000-0001-6357-8115
person.identifier.orcid0000-0003-1613-9675
person.identifier.orcid0000-0003-4936-1301
person.identifier.orcid0000-0001-8608-0248
person.identifier.orcid0000-0002-3096-8325
person.identifier.ridE-5876-2013
person.identifier.ridK-1835-2013
person.identifier.ridL-3104-2014
person.identifier.scopus-author-id35734956500
person.identifier.scopus-author-id26658759200
person.identifier.scopus-author-id7003557499
person.identifier.scopus-author-id6701760475
person.identifier.scopus-author-id7003705645
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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