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ESBL and AmpC β-Lactamases in Clinical Strains of Escherichia coli from Serra da Estrela, Portugal

2019Journal article Open access
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dc.contributor.authorOliveira, Cátia Marlene Meneses
dc.contributor.authorAmador, Paula
dc.contributor.authorPrudêncio, C.
dc.contributor.authorTomaz, C. T.
dc.contributor.authorRatado, Paulo
dc.contributor.authorFernandes, Ruben
dc.date.accessioned2020-02-11T17:25:25Z
dc.date.available2020-02-11T17:25:25Z
dc.date.issued2019
dc.description.abstractBackground and Objectives: Given the considerable spatial, temporal, and ecological factors, heterogeneity, which affects emergency response, persistence, and dissemination of genetic determinants that confer microorganisms their resistance to antibiotics, several authors claim that antibiotics' resistance must be perceived as an ecological problem. The aim of this study was to determine the prevalence of broad-spectrum bla genes, not only Extended-spectrum β-lactamases (ESBL) but also AmpC-types, in clinical strains of Escherichia coli isolated from Portugal (in the highest region of the country, Serra da Estrela) to disclose susceptibility profiles among different genotypes, and to compare the distribution of bla genes expressing broad-spectrum enzymes. Materials and Methods: Clinical strains of Escherichia coli presenting resistance to third generation (3G) cephalosporins and susceptibility to inhibition by clavulanic acid were studied by means of phenotypic and molecular profiling techniques for encoding β-lactamases genes. Results: Strains were mainly isolated from hospital populations (97%). Molecular analysis enabled the detection of 49 bla genes, in which 55% (27/49) were identified as blaOXA-1-like, 33% (16/49) as blaCTX-M-group-1, 10% (5/49) as blaTEM, and 2% (1/49) were identified as genes blaCIT (AmpC). Among all blaOXA-1-like detected, about 59% of strains expressed at least another bla gene. Co-production of β-lactamases was observed in 40% of strains, with the co-production of CTX-M group 1 and OXA-1-like occurring as the most frequent. Conclusions: This is the first study using microorganisms isolated from native people from the highest Portuguese mountain regions, showing an unprecedent high prevalence of genes blaOXA-1-like in this country.pt_PT
dc.description.sponsorshipNORTE-01-0145-FEDER-000012; SAICT-POL/24325/2016.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/medicina55060272pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/9221
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBacterial Proteinspt_PT
dc.subjectCephalosporinspt_PT
dc.subjectDrug Resistance, Microbialpt_PT
dc.subjectEscherichia colipt_PT
dc.subjectEscherichia coli Infectionspt_PT
dc.subjectbeta-Lactamasespt_PT
dc.titleESBL and AmpC β-Lactamases in Clinical Strains of Escherichia coli from Serra da Estrela, Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F04293%2F2013/PT
oaire.citation.issue6pt_PT
oaire.citation.startPage272pt_PT
oaire.citation.titleMedicinapt_PT
oaire.citation.volume55pt_PT
oaire.fundingStream5876
person.familyNameAmador
person.familyNamePrudêncio
person.familyNameTomaz
person.familyNameRatado
person.familyNameFernandes
person.givenNamePaula
person.givenNameCristina
person.givenNameCândida
person.givenNamePaulo
person.givenNameRuben
person.identifier1323406
person.identifier1200571
person.identifier2198714
person.identifier.ciencia-id601E-08F7-7729
person.identifier.ciencia-idC81E-F4EE-FADE
person.identifier.ciencia-idFE13-0C44-13D1
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.orcid0000-0002-5228-205X
person.identifier.orcid0000-0002-9920-936X
person.identifier.orcid0000-0002-0927-2331
person.identifier.orcid0000-0002-5976-0181
person.identifier.orcid0000-0001-8933-3984
person.identifier.ridE-8029-2015
person.identifier.ridF-9367-2014
person.identifier.scopus-author-id26647421600
person.identifier.scopus-author-id6508057930
person.identifier.scopus-author-id7004546303
person.identifier.scopus-author-id24399749400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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