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Isolation and culture of human umbilical artery smooth muscle cells expressing functional calcium channels

2009-01-01Journal article Restricted access
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dc.contributor.authorCairrão, Elisa
dc.contributor.authorSantos-Silva, António
dc.contributor.authorAlvarez, Ezequiel
dc.contributor.authorCorreia, Ilídio Joaquim Sobreira
dc.contributor.authorVerde, Ignacio
dc.date.accessioned2018-03-15T16:00:29Z
dc.date.available2018-03-15T16:00:29Z
dc.date.issued2009-01-01
dc.description.abstractThe human umbilical cord is a biological sample that can be easily obtained just after birth. A methodology was developed to perform cultures of human umbilical artery smooth muscle cells (HUASMC) expressing contractile proteins and functional ionic channels. To avoid fibroblast and endothelial cell contamination, we mechanically separated the tunica media, which only contains HUASMC and matrix proteins. To isolate the cells, collagenase V and elastase were used as hydrolyzing enzymes. The isolated cells were plated in collagen-coated dishes to obtain cultures of HUASMC. The cells obtained after different passages (1 to 6) exhibit the characteristic vascular smooth cell morphology and express smooth muscle alpha-2 actin, myosin heavy chain SM1, and alpha subunits of L- and T-type calcium channels (Cav 1.2, Cav 1.2, and Cav 3.2). Electrophysiology recordings for L- and T-type calcium channels were made, indicating that these channels are functional in the cultured cells. In conclusion, the procedure developed allows obtaining cultures of HUASMC expressing contractile proteins and also functional ionic channels. These cells could be used to study cellular and molecular aspects about the regulation of the vascular function.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCairrão, E., Santos-Silva, A.J., Alvarez, E., Correia, I.J. e Verde, I. (2009) “Isolation and culture of human umbilical artery smooth muscle cells expressing functional calcium channels”, In Vitro Cellular & Developmental Biology – Animal, Vol. 45 (3), pp. 175-184pt_PT
dc.identifier.doi10.1007/s11626-008-9161-6pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4622
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationSTUDY OF THE REGULATION OF THE DIFFERENT TYPES OF CALCIUM CHANNELS IMPLICATED IN VASCULAR SMOOTH MUSCLE CONTRACTILITY
dc.relationESTUDOS ESTRUTURAIS, MECANÍSTICOS E FUNCIONAIS DE CANAIS DE CÁLCIO MEMBRANARES
dc.relation.publisherversionhttps://link.springer.com/article/10.1007%2Fs11626-008-9161-6pt_PT
dc.subjectUmbilical arterypt_PT
dc.subjectSmooth muscle cellspt_PT
dc.subjectPatch-clamppt_PT
dc.subjectCalcium channelspt_PT
dc.titleIsolation and culture of human umbilical artery smooth muscle cells expressing functional calcium channelspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSTUDY OF THE REGULATION OF THE DIFFERENT TYPES OF CALCIUM CHANNELS IMPLICATED IN VASCULAR SMOOTH MUSCLE CONTRACTILITY
oaire.awardTitleESTUDOS ESTRUTURAIS, MECANÍSTICOS E FUNCIONAIS DE CANAIS DE CÁLCIO MEMBRANARES
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBDE%2F15532%2F2004/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F14458%2F2003/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F19776%2F2004/PT
oaire.citation.endPage184pt_PT
oaire.citation.startPage175pt_PT
oaire.citation.titleIn Vitro Cellular & Developmental Biology - Animalpt_PT
oaire.citation.volume45pt_PT
oaire.fundingStreamSFRH
person.familyNameCairrao Rodrigues Oliveira
person.familyNameJoaquim Sobreira Correia
person.familyNameVerde
person.givenNameMaria Elisa
person.givenNameIlídio
person.givenNameIgnacio
person.identifierAAB-1164-2020
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-id0611-71B8-191E
person.identifier.ciencia-idF610-7373-DC81
person.identifier.ciencia-idD012-1D7C-791A
person.identifier.orcid0000-0002-4823-5701
person.identifier.orcid0000-0003-1613-9675
person.identifier.orcid0000-0003-3492-5725
person.identifier.ridM-3991-2013
person.identifier.scopus-author-idScopus Author ID: 23476537500
person.identifier.scopus-author-id7003557499
person.identifier.scopus-author-id55913729400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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