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Development of dual-crosslinked Pluronic F127/Chitosan injectable hydrogels incorporating graphene nanosystems for breast cancer photothermal therapy and antibacterial applications

2024-08-28Journal article Open access
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dc.contributor.authorPouso, Manuel António do Rosário
dc.contributor.authorMelo, Bruna L.
dc.contributor.authorGonçalves, Joaquim
dc.contributor.authorMendonça, António
dc.contributor.authorCorreia, I.J.
dc.contributor.authorDiogo, Duarte de Melo
dc.date.accessioned2024-09-17T11:00:22Z
dc.date.available2024-09-17T11:00:22Z
dc.date.issued2024-08-28
dc.description.abstractNanomaterials with responsiveness to near-infrared light can mediate the photoablation of cancer cells with an exceptional spatio-temporal resolution. However, the therapeutic outcome of this modality is limited by the nanostructures’ poor tumor uptake. To address this bottleneck, it is appealing to develop injectable in situ forming hydrogels due to their capacity to perform a tumor-confined delivery of the nanomaterials with minimal off-target leakage. In particular, injectable in situ forming hydrogels based on Pluronic F127 have been emerging due to their FDA-approval status, biocompatibility, and thermosensitive sol–gel transition. Nevertheless, the application of Pluronic F127 hydrogels has been limited due to their fast dissociation in aqueous media. Such limitation may be addressed by combining the thermoresponsive sol–gel transition of Pluronic F127 with other polymers with crosslinking capabilities. In this work, a novel dual-crosslinked injectable in situ forming hydrogel based on Pluronic F127 (thermosensitive gelation) and Chitosan (ionotropic gelation in the presence of NaHCO3), loaded with Dopamine-reduced graphene oxide (DOPA-rGO; photothermal nanoagent), was developed for application in breast cancer photothermal therapy. The dual-crosslinked hydrogel incorporating DOPA-rGO showed a good injectability (through 21 G needles), in situ gelation capacity and cytocompatibility (viability > 73 %). As importantly, the dual-crosslinking improved the hydrogel’s porosity and prevented its premature degradation. After irradiation with near-infrared light, the dual-crosslinked hydrogel incorporating DOPA-rGO produced a photothermal heating (ΔT ≈ 22 °C) that reduced the breast cancer cells’ viability to just 32 %. In addition, this formulation also demonstrated a good antibacterial activity by reducing the viability of S. aureus and E. coli to 24 and 33 %, respectively. Overall, the dual-crosslinked hydrogel incorporating DOPA-rGO is a promising macroscale technology for breast cancer photothermal therapy and antimicrobial applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.ejpb.2024.114476pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/14512
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.relationSilk Sericin as an industrial wastewater with valuable biomedical potential
dc.relationAssociate Laboratory of Energy, Transports and Aerospace.
dc.relationAssociate Laboratory of Energy, Transports and Aeronautics
dc.relationShear-thinning and self-healing hydrogels: tuning the polymer-related features for the tumor confined delivery of therapeutics aimed for metastatic breast cancer immuno-photothermal therapy
dc.relationThiol-ene click injectable in situ forming hydrogels incorporating graphene: optimization of the polymer-related features for the immuno-photothermal therapy of metastatic breast cancer
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0939641124003023?pes=vor#ak005pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAntimicrobial applicationspt_PT
dc.subjectCancer therapypt_PT
dc.subjectChitosan hydrogelspt_PT
dc.subjectInjectable hydrogelspt_PT
dc.subjectPhotothermal therapypt_PT
dc.subjectPluronic F127 hydrogelspt_PT
dc.subjectReduced graphene oxidept_PT
dc.titleDevelopment of dual-crosslinked Pluronic F127/Chitosan injectable hydrogels incorporating graphene nanosystems for breast cancer photothermal therapy and antibacterial applicationspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleSilk Sericin as an industrial wastewater with valuable biomedical potential
oaire.awardTitleAssociate Laboratory of Energy, Transports and Aerospace.
oaire.awardTitleAssociate Laboratory of Energy, Transports and Aeronautics
oaire.awardTitleShear-thinning and self-healing hydrogels: tuning the polymer-related features for the tumor confined delivery of therapeutics aimed for metastatic breast cancer immuno-photothermal therapy
oaire.awardTitleThiol-ene click injectable in situ forming hydrogels incorporating graphene: optimization of the polymer-related features for the immuno-photothermal therapy of metastatic breast cancer
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTA-BTA%2F0696%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0079%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50022%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND4ed/2021.00590.CEECIND%2FCP1661%2FCT0001/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de Projetos de I&D em Todos os Domínios Científicos - 2022/2022.06320.PTDC/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/2021.06044.BD/PT
oaire.citation.conferencePlaceNetherlandpt_PT
oaire.citation.titleEuropean Journal of Pharmaceutics and Biopharmaceuticspt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND4ed
oaire.fundingStreamConcurso de Projetos de I&D em Todos os Domínios Científicos - 2022
oaire.fundingStreamPOR_CENTRO
person.familyNamedo Rosário Pouso
person.familyNameLopes Melo
person.familyNameGonçalves
person.familyNameMendonça
person.familyNameJoaquim Sobreira Correia
person.familyNameMiguel de Melo Diogo
person.givenNameManuel António
person.givenNameBruna Daniela
person.givenNameJoaquim
person.givenNameAntónio
person.givenNameIlídio
person.givenNameDuarte
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person.identifier.ciencia-idA613-7659-3BA9
person.identifier.ciencia-id2419-6E20-F958
person.identifier.ciencia-idF610-7373-DC81
person.identifier.ciencia-id9C10-9BD8-634E
person.identifier.orcid0000-0001-9816-6284
person.identifier.orcid0000-0002-0078-1877
person.identifier.orcid0009-0008-2314-701X
person.identifier.orcid0000-0001-8069-0440
person.identifier.orcid0000-0003-1613-9675
person.identifier.orcid0000-0001-9984-3603
person.identifier.ridI-3093-2012
person.identifier.scopus-author-id56890866600
person.identifier.scopus-author-id7003557499
person.identifier.scopus-author-id56266511300
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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