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Formulation of chitosan–TPP–pDNA nanocapsules for gene therapy applications

2010-12-06Journal article Restricted access
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dc.contributor.authorGaspar, Vítor Manuel Abreu
dc.contributor.authorSousa, Fani
dc.contributor.authorQueiroz, João
dc.contributor.authorCorreia, Ilídio Joaquim Sobreira
dc.date.accessioned2018-03-19T10:41:26Z
dc.date.available2018-03-19T10:41:26Z
dc.date.issued2010-12-06
dc.description.abstractThe encapsulation of DNA inside nanoparticles meant for gene delivery applications is a challenging process where several parameters need to be modulated in order to design nanocapsules with specific tailored characteristics. The purpose of this study was to investigate and improve the formulation parameters of plasmid DNA (pDNA) loaded in chitosan nanocapsules using tripolyphosphate (TPP) as polyanionic crosslinker. Nanocapsule morphology and encapsulation efficiency were analyzed as a function of chitosan degree of deacetylation and chitosan–TPP ratio. The manipulation of these parameters influenced not only the particle size but also the encapsulation and release of pDNA. Consequently the transfection efficiency of the nanoparticulated systems was also enhanced with the optimization of the particle characteristics. Overall, the differently formulated nanoparticulated systems possess singular properties that can be employed according to the desired gene delivery application.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGaspar, V.M., Sousa, F., Queiroz, J.A. e Correia, I.J. (2011) ”Formulation of chitosan-TPP-pDNA nanocapsules for gene therapy applications” Nanotechnology, Vol. 22(1), nº do artigo 015101pt_PT
dc.identifier.doi10.1088/0957-4484/22/1/015101pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4628
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherIOPSCIENCEpt_PT
dc.relation.publisherversionhttp://iopscience.iop.org/article/10.1088/0957-4484/22/1/015101/metapt_PT
dc.subjectPDNApt_PT
dc.subjectGene deliverypt_PT
dc.subjectNanocapsulespt_PT
dc.titleFormulation of chitosan–TPP–pDNA nanocapsules for gene therapy applicationspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage015101pt_PT
oaire.citation.titleNanotechnologypt_PT
oaire.citation.volume22pt_PT
person.familyNameGaspar
person.familyNameSousa
person.familyNameQueiroz
person.familyNameCorreia
person.givenNameVítor
person.givenNameFani
person.givenNameJoão
person.givenNameIlídio Joaquim Sobreira
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-id6F16-3640-73E3
person.identifier.ciencia-id991D-2E13-A840
person.identifier.ciencia-id931E-B66D-E341
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0002-0372-2493
person.identifier.orcid0000-0001-9996-2194
person.identifier.orcid0000-0002-3096-8325
person.identifier.orcid0000-0003-1613-9675
person.identifier.ridB-1602-2017
person.identifier.ridA-2014-2017
person.identifier.ridL-3104-2014
person.identifier.scopus-author-id36968590900
person.identifier.scopus-author-id7005110268
person.identifier.scopus-author-id7003705645
person.identifier.scopus-author-id7003557499
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3eeac15e-ebf4-4dc2-aeb8-698087d6d2fc
relation.isAuthorOfPublication2935de97-fc90-4d38-9505-4caa364f8a10
relation.isAuthorOfPublicationc798c033-c160-4f99-a8ad-04416923943f
relation.isAuthorOfPublicationf2c3e779-34a2-4309-950a-80687664c6ad
relation.isAuthorOfPublication.latestForDiscovery3eeac15e-ebf4-4dc2-aeb8-698087d6d2fc

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