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Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri

2021Journal article Open access
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dc.contributor.authorBonifácio, Marina
dc.contributor.authorMateus, Cristiana
dc.contributor.authorAlves, Ana R.
dc.contributor.authorMaldonado, Emanuel
dc.contributor.authorDuarte, Ana Paula
dc.contributor.authorDomingues, Fernanda
dc.contributor.authorOleastro, Mónica
dc.contributor.authorFerreira, Susana
dc.date.accessioned2023-11-16T15:40:14Z
dc.date.available2023-11-16T15:40:14Z
dc.date.issued2021
dc.description.abstractAliarcobacter butzleri is an emergent enteropathogen, showing high genetic diversity, which likely contributes to its adaptive capacity to different environments. Whether natural transformation can be a mechanism that generates genetic diversity in A. butzleri is still unknown. In the present study, we aimed to establish if A. butzleri is naturally competent for transformation and to investigate the factors influencing this process. Two different transformation procedures were tested using exogenous and isogenic DNA containing antibiotic resistance markers, and different external conditions influencing the process were evaluated. The highest number of transformable A. butzleri strains were obtained with the agar transformation method when compared to the biphasic system (65% versus 47%). A. butzleri was able to uptake isogenic chromosomal DNA at different growth phases, and the competence state was maintained from the exponential to the stationary phases. Overall, the optimal conditions for transformation with the biphasic system were the use of 1 µg of isogenic DNA and incubation at 30 ◦C under a microaerobic atmosphere, resulting in a transformation frequency ~8 × 10−6 transformants/CFU. We also observed that A. butzleri favored the transformation with the genetic material of its own strain/species, with the DNA incorporation process occurring promptly after the addition of genomic material. In addition, we observed that A. butzleri strains could exchange genetic material in co-culture assays. The presence of homologs of well-known genes involved in the competence in the A. butzleri genome corroborates the natural competence of this species. In conclusion, our results show that A. butzleri is a naturally transformable species, suggesting that horizontal gene transfer mediated by natural transformation is one of the processes contributing to its genetic diversity. In addition, natural transformation can be used as a tool for genetic studies of this species.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/pathogens10070909pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/13695
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationHealth Sciences Research Centre
dc.relationUnravelling the virulence potential of Aliarcobacter butzleri
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAliarcobacter butzleript_PT
dc.subjectNatural competencept_PT
dc.subjectTransformationpt_PT
dc.titleNatural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleript_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleUnravelling the virulence potential of Aliarcobacter butzleri
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//UI%2FBD%2F151023%2F2021/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL57%2F2016%2FCP1334%2FCP1644%2FCT0001/PT
oaire.citation.issue7pt_PT
oaire.citation.startPage909pt_PT
oaire.citation.titlePathogenspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamDL 57/2016
person.familyNameLopes Mateus
person.familyNameMaldonado
person.familyNameDuarte
person.familyNameDomingues
person.familyNameOleastro
person.familyNameFerreira
person.givenNameCristiana
person.givenNameEmanuel Filipe Escaleira
person.givenNameAna Paula
person.givenNameFernanda
person.givenNameMónica
person.givenNameSusana
person.identifierrrpPC6EAAAAJ
person.identifier1255684
person.identifier269435
person.identifier.ciencia-id591D-CAC9-F991
person.identifier.ciencia-idE311-80D2-8029
person.identifier.ciencia-idBC15-D376-80FE
person.identifier.ciencia-id6514-0498-3E8F
person.identifier.ciencia-id4115-04A9-FBCB
person.identifier.ciencia-idF718-3569-E911
person.identifier.orcid0000-0003-1303-0295
person.identifier.orcid0000-0002-0084-6116
person.identifier.orcid0000-0003-3333-5977
person.identifier.orcid0000-0002-9540-0853
person.identifier.orcid0000-0001-6360-2576
person.identifier.orcid0000-0001-8308-2862
person.identifier.ridB-5904-2009
person.identifier.ridN-7295-2013
person.identifier.ridA-6810-2016
person.identifier.scopus-author-id7102602131
person.identifier.scopus-author-id10139481400
person.identifier.scopus-author-id6603067224
person.identifier.scopus-author-id34874668400
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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