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Multimodal ionic liquid-based chromatographic supports for an effective RNA purification

2023-03-02Artigo científico Acesso aberto
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datacite.subject.fosCiências Médicas::Ciências da Saúde
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorCarapito, Ana Rita
dc.contributor.authorBernardo, Sandra C.
dc.contributor.authorPereira, Matheus M.
dc.contributor.authorNeves, Márcia C.
dc.contributor.authorFreire, Mara
dc.contributor.authorSousa, Fani
dc.date.accessioned2026-02-06T15:35:54Z
dc.date.available2026-02-06T15:35:54Z
dc.date.issued2023-03-02
dc.description.abstractNucleic acids have been considered interesting molecules to be used as biopharmaceuticals for the treatment of various diseases, in gene therapy strategies. In particular, RNA arises as the most promising approach because it does not require access to the nucleus of cells to exert its function; however, it is quite challenging due to its labile nature. To increase the possibility of translating RNA-based technology to clinical protocols, the biomanufacturing of RNAs has been intensively exploited in the last few years. However, the standard RNA purification processes remain time-consuming and present limitations regarding recovery yield and purity. This work describes the functionalization of chromatographic silica-based supports with four ionic liquids (ILs) composed of functional moieties that can promote distinct interactions with nucleic acids. After an initial screening to evaluate the binding and elution behavior of nucleic acids in the IL-based supports, SSi[C3C3NH2Im]Cl has shown to be the most promising for further purification assays. This support was studied for the RNA purification from different samples (clarified or more complex) and has shown to be highly effective, for all the conditions studied. Generally, it is here presented a new method for RNA isolation in a single step, using an IL-based chromatographic support, able to eliminate the usage of hazardous compounds often included in standard RNA extraction protocols.eng
dc.identifier.citationRita Carapito; Sandra C. Bernardo; Matheus M. Pereira; Márcia C. Neves; Mara G. Freire; Fani Sousa. "Multimodal ionic liquid-based chromatographic supports for an effective RNA purification". Separation and Purification Technology (2023): https://doi.org/10.1016/j.seppur.2023.123676
dc.identifier.doi10.1016/j.seppur.2023.123676
dc.identifier.issn1873-3794
dc.identifier.urihttp://hdl.handle.net/10400.6/19868
dc.language.isoeng
dc.peerreviewedyes
dc.publisherELSEVIER
dc.relationHealth Sciences Research Centre [UIDB/00709/2020]
dc.relationCICS-UBI [UIDP/00709/2020]
dc.relationSuportes modificados com Liquidos Iónicos para a Purificação de pré-miRNAs envolvidos na doença de Alzheimer [PTDC/BII-BBF/29496/2017]
dc.relationCICECO-Aveiro Institute of Materials [UIDB/50011/2020]
dc.relationCICECO-Aveiro Institute of Materials [UIDP/50011/2020]
dc.relationCICECO – Aveiro Institute of Materials (LA/P/0006/2020)
dc.relationInnovative continuous mode purification of pre-miR-29b biopharmaceuticals from Rhodovulum sulfidophilum extract
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S1383586623005841?via%3Dihub
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectNucleic acids
dc.subjectIonic liquid
dc.subjectIonic liquid-based support
dc.subjectMultimodal ligand
dc.subjectPurification
dc.titleMultimodal ionic liquid-based chromatographic supports for an effective RNA purificationpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre [UIDB/00709/2020]
oaire.awardTitleCICS-UBI [UIDP/00709/2020]
oaire.awardTitleSuportes modificados com Liquidos Iónicos para a Purificação de pré-miRNAs envolvidos na doença de Alzheimer [PTDC/BII-BBF/29496/2017]
oaire.awardTitleCICECO-Aveiro Institute of Materials [UIDB/50011/2020]
oaire.awardTitleCICECO-Aveiro Institute of Materials [UIDP/50011/2020]
oaire.awardTitleCICECO – Aveiro Institute of Materials (LA/P/0006/2020)
oaire.awardTitleInnovative continuous mode purification of pre-miR-29b biopharmaceuticals from Rhodovulum sulfidophilum extract
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Programático/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBII-BBF%2F29496%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50011%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50011%2F2020/PT
oaire.awardURIhttp://hdl.handle.net/10400.6/19865
oaire.awardURIhttp://hdl.handle.net/10400.6/19867
oaire.citation.issue123676
oaire.citation.titleSeparation and Purification Technology
oaire.citation.volume315
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Programático
oaire.fundingStream9471 - RIDTI
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCarapito
person.familyNameda Silva Bernardo
person.familyNameM. Pereira
person.familyNameNeves
person.familyNameFreire
person.familyNameSousa
person.givenNameAna Rita Mugeiro
person.givenNameSandra Cristina
person.givenNameMatheus
person.givenNameMárcia C.
person.givenNameMara
person.givenNameFani
person.identifier1342598
person.identifier.ciencia-id8E11-013D-1419
person.identifier.ciencia-id0616-8D07-D7B0
person.identifier.ciencia-id3C1F-F5A0-205C
person.identifier.ciencia-id411A-AD38-6EB1
person.identifier.ciencia-id6718-1145-692F
person.identifier.ciencia-id991D-2E13-A840
person.identifier.orcid0000-0003-2872-3609
person.identifier.orcid0000-0003-3524-6900
person.identifier.orcid0000-0001-5611-4080
person.identifier.orcid0000-0001-8895-0614
person.identifier.orcid0000-0001-9996-2194
person.identifier.ridGRF-0544-2022
person.identifier.ridB-8458-2008
person.identifier.ridA-2014-2017
person.identifier.scopus-author-id56096325200
person.identifier.scopus-author-id7103415227
person.identifier.scopus-author-id7102299235
person.identifier.scopus-author-id7005110268
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
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project.funder.nameFundação para a Ciência e a Tecnologia
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