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Effect of extracellular calcium on regucalcin expression and cell viability in neoplastic and non-neoplastic human prostate cells

2015-10Journal article Restricted access
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dc.contributor.authorVaz, Cátia
dc.contributor.authorRodrigues, Daniel B
dc.contributor.authorSocorro, Sílvia
dc.contributor.authorBaptista, Cláudio
dc.date.accessioned2019-12-04T17:04:43Z
dc.date.available2019-12-04T17:04:43Z
dc.date.issued2015-10
dc.description.abstractExtracellular calcium (Ca2+o) and its receptor, the Ca2+-sensing receptor (CaSR), play an important role in prostate physiology, and it has been shown that the deregulation of Ca2+ homeostasis and the overexpression of CaSR are involved in prostate cancer (PCa). Regucalcin (RGN), a Ca2+-binding protein that plays a relevant role in intracellular Ca2+ homeostasis, was identified as an under-expressed protein in human PCa. Moreover, RGN was associated with suppression of cell proliferation, suggesting that the loss of RGN may favor development and progression of PCa. This work aims to unveil the role of Ca2+o on RGN expression and viability of non-neoplastic (PNT1A) and neoplastic (LNCaP) prostate cell lines. It was demonstrated that Ca2+o up-regulates RGN expression in both cell lines, but important differences were found between cells for dose- and time-responses to Ca2+o treatment. It was also shown that high [Ca2+]o triggers different effects on cell proliferation of neoplastic and non-neoplastic PCa cells, which seems to be related with RGN expression levels. This suggests the involvement of RGN in the regulation of cell proliferation in response to Ca2+o treatment. Also, the effect of Ca2+o on CaSR expression seems to be dependent of RGN expression, which is strengthened by the fact that RGN-knockdown in PNT1A cells increases the CaSR expression, whereas transgenic rats overexpressing RGN exhibit low levels of CaSR. Overall, our results highlighted the importance of RGN as a regulatory protein in Ca2+-dependent signaling pathways and its deregulation of RGN expression by Ca2+o may contribute for onset and progression of PCa.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.bbamcr.2015.07.006pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/7663
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationROLE OF REGUCALCIN IN BREAST CELLS PROLIFERATION AND TUMOR DEVELOPMENT
dc.subjectPNT1Apt_PT
dc.subjectLNCaPpt_PT
dc.subjectProstate cancerpt_PT
dc.subjectRegucalcinpt_PT
dc.subjectCalciumpt_PT
dc.subjectCaSRpt_PT
dc.titleEffect of extracellular calcium on regucalcin expression and cell viability in neoplastic and non-neoplastic human prostate cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleROLE OF REGUCALCIN IN BREAST CELLS PROLIFERATION AND TUMOR DEVELOPMENT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FSAU%2FUI0709%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F70316%2F2010/PT
oaire.citation.endPage2628pt_PT
oaire.citation.issue10 Pt Apt_PT
oaire.citation.startPage2621pt_PT
oaire.citation.titleBBA - Biochimica et Biophysica Actapt_PT
oaire.citation.volume1853pt_PT
oaire.fundingStream5876
person.familyNameVaz
person.familyNameSocorro
person.familyNameMaia Baptista
person.givenNameCátia
person.givenNameSílvia Cristina da Cruz Marques
person.givenNameCláudio Jorge
person.identifierhttps://scholar.google.pt/citations?user=PA3L_h8AAAAJ&hl=pt-PT
person.identifierhttps://scholar.google.pt/citations?user=hFKOd_QAAAAJ&hl=pt-PT&oi=ao
person.identifier.ciencia-id1C1D-F91F-D937
person.identifier.ciencia-idAB17-5C25-1F2C
person.identifier.ciencia-id5115-FCBD-A31F
person.identifier.orcid0000-0002-7720-4234
person.identifier.orcid0000-0001-8181-488X
person.identifier.orcid0000-0002-5658-5445
person.identifier.scopus-author-id23097994600
person.identifier.scopus-author-id23097426600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicação.pt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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