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Comparative study of sample preparation procedures to determine the main compounds in ayahuasca beverages by QuEChERS and high‐performance liquid chromatography analysis

2024-04-13Journal article Open access
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dc.contributor.authorGonçalves, Joana
dc.contributor.authorRosado, Tiago
dc.contributor.authorBarroso, Mário
dc.contributor.authorRestolho, José
dc.contributor.authorFernández, Nicolás
dc.contributor.authorLuís, Ângelo
dc.contributor.authorGallardo, Eugenia
dc.contributor.authorDuarte, Ana Paula
dc.date.accessioned2024-06-17T10:10:42Z
dc.date.available2024-06-17T10:10:42Z
dc.date.issued2024-04-13
dc.description.abstractIntroduction Ayahuasca is a psychoactive drink originally consumed by indigenous people of the Amazon. The lack of regulation of this drink leads to uncontrolled consumption, and it is often consumed in religious contexts. Objective The aim of this work is to compare three miniaturised extraction techniques for extracting the main ayahuasca compounds from beverages. Methodology Three sample pretreatment techniques were evaluated (dispersive liquid–liquid microextraction [DLLME], microextraction by packed sorbent [MEPS] and QuEChERS [Quick, Easy, Cheap, Effective, Rugged and Safe]) for the simultaneous extraction of N,N-dimethyltryptamine (DMT), tetrahydroharmine (THH), harmine, harmaline, harmol and harmalol from ayahuasca beverage samples. Then, the most promising technique (QuEChERS) was chosen to pre-concentrate the analytes, subsequently detected by high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD). Results The procedure was optimised, with the final conditions being 500 μL of extractor solvent, 85 mg of primary secondary amine (PSA) and 4 s of vortexing. The analytical method was validated, showing to be linear between 0.16 and 10 μg/mL for β-carbolines and between 0.016 and 1 μg/mL for DMT, with coefficients of determination (R2) between 0.9968 and 0.9993. The limit of detection (LOD) and lower limit of quantification (LLOQ) were 0.16 μg/mL for all compounds, except for DMT (0.016 μg/mL) and extraction efficiencies varied between 60.2% and 88.0%. Conclusion The analytical methodology proved to be accurate and precise, with good linearity, LODs and LLOQs. This method has been fully validated and successfully applied to ayahuasca beverage samples.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/pca.3370pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/14436
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.relationalterado para: “Avaliação in vitro associados ao consumo de Ayahuasca” AVALIAÇÃO IN VITRO DOS EFEITOS TOXICOLÓGICOS ASSOCIADOS AO CONSUMO DE AYAHUASCA.
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAyahuascapt_PT
dc.subjectDispersive Liquid–Liquid Microextraction - DLLMEpt_PT
dc.subjectMicroextraction by Packed Sorbent - MEPSpt_PT
dc.subjectQuEChERSpt_PT
dc.titleComparative study of sample preparation procedures to determine the main compounds in ayahuasca beverages by QuEChERS and high‐performance liquid chromatography analysispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitlealterado para: “Avaliação in vitro associados ao consumo de Ayahuasca” AVALIAÇÃO IN VITRO DOS EFEITOS TOXICOLÓGICOS ASSOCIADOS AO CONSUMO DE AYAHUASCA.
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F149360%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL 57%2F2016%2FCP1354%2FCT0010/PT
oaire.citation.titlePhytochemical Analysispt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_CENTRO
oaire.fundingStreamDL 57/2016
person.familyNameGonçalves
person.familyNameRosado
person.familyNameBarroso
person.familyNameRestolho
person.familyNameFernández
person.familyNameSantos Luís
person.familyNameGallardo
person.familyNameDuarte
person.givenNameJoana
person.givenNameTiago
person.givenNameMário
person.givenNameJosé
person.givenNameNicolás
person.givenNameÂngelo Filipe
person.givenNameEugenia
person.givenNameAna Paula
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person.identifier.ridB-6330-2013
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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