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Manufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineering

2014-03-21Journal article Restricted access
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dc.contributor.authorCarlos, Gabriela Soares Diogo
dc.contributor.authorGaspar, Vítor Manuel Abreu
dc.contributor.authorSerra, Inês Raquel Tavares
dc.contributor.authorFradique, Ricardo Gil
dc.contributor.authorCorreia, Ilídio Joaquim Sobreira
dc.date.accessioned2018-03-20T10:40:17Z
dc.date.available2018-03-20T10:40:17Z
dc.date.issued2014-03-21
dc.description.abstractThe growing need to treat bone-related diseases in an elderly population compels the development of novel bone substitutes to improve patient quality of life. In this context, the advent of affordable and effective rapid prototyping equipment, such as the Fab@home plotter, has contributed to the development of novel scaffolds for bone tissue engineering. In this study, we report for the first time the use of a Fab@home plotter for the production of 3D scaffolds composed by beta-tricalcium phosphate (β-TCP)/alginate hybrid materials. β-TCP/alginate mixtures were used in a proportion of 50/50% (w/w), 30/70% (w/w) and 20/80% (w/w). The printing parameters were optimized to a nozzle diameter of 20 Gauge for the production of rigid scaffolds with pre-defined architectures. We observed that, despite using similar printing parameters, both the precision and resolution of the scaffolds were significantly affected by the blend's viscosity. In particular, we demonstrate that the higher viscosity of 50/50 scaffolds (150.0 ± 3.91 mPa s) provides a higher precision in the extrusion process. The physicochemical and biological characterization of the samples demonstrated that the 50/50 scaffolds possessed a resistance to compression comparable to that of native trabecular bone. Moreover, this particular formulation also exhibited a Young's modulus that was higher than that of trabecular bone. Scanning electron microscopy and fluorescence microscopy analysis revealed that osteoblasts were able to adhere, proliferate and also penetrate into the scaffold's architecture. Altogether, our findings suggest that the Fab@home printer can be employed in the manufacture of reproducible scaffolds, using a formulation 50/50 alginate-β-TCP that has suitable properties to be applied as bone substitutes in the future.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDiogo, G.S., Gaspar, V.M., Serra, I.R., Fradique, R. e Correia, I.J. (2014) "Manufacture of β-TCP/Alginate scaffolds through a Fab@home model for application in bone tissue engineering", Biofabrication, Vol. 6 (2), pp. 025001pt_PT
dc.identifier.doi10.1088/1758-5082/6/2/025001pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/4651
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherIOP Publishingpt_PT
dc.relationIsolation and Purification of Plasmid DNA for Cancer Therapy
dc.relationStrategic Project - UI 709 - 2011-2012
dc.relationBIOSYNTHESIS AND PURIFICATION OF MINICIRCLE DNA FOR APPLICATION IN DIABETES CELL-SPECIFIC GENE THERAPY
dc.relation.publisherversionhttp://iopscience.iop.org/article/10.1088/1758-5082/6/2/025001/metapt_PT
dc.subjectRapid prototypingpt_PT
dc.subjectBone tissue engineeringpt_PT
dc.subjectBiopolymerspt_PT
dc.subject3D plotterpt_PT
dc.subjectHybrid materialspt_PT
dc.titleManufacture of β-TCP/alginate scaffolds through a Fab@home model for application in bone tissue engineeringpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleIsolation and Purification of Plasmid DNA for Cancer Therapy
oaire.awardTitleStrategic Project - UI 709 - 2011-2012
oaire.awardTitleBIOSYNTHESIS AND PURIFICATION OF MINICIRCLE DNA FOR APPLICATION IN DIABETES CELL-SPECIFIC GENE THERAPY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FEBB-BIO%2F114320%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FUI0709%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F80402%2F2011/PT
oaire.citation.startPage025001pt_PT
oaire.citation.titleBiofabricationpt_PT
oaire.citation.volume6pt_PT
oaire.fundingStream5876-PPCDTI
oaire.fundingStream6820 - DCRRNI ID
oaire.fundingStreamFARH
person.familyNameDiogo
person.familyNameGaspar
person.familyNameFradique
person.familyNameJoaquim Sobreira Correia
person.givenNameGabriela
person.givenNameVítor
person.givenNameRicardo
person.givenNameIlídio
person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-id6F16-3640-73E3
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0002-5696-631X
person.identifier.orcid0000-0002-0372-2493
person.identifier.orcid0000-0001-6331-2034
person.identifier.orcid0000-0003-1613-9675
person.identifier.ridB-1602-2017
person.identifier.scopus-author-id36968590900
person.identifier.scopus-author-id55797637100
person.identifier.scopus-author-id7003557499
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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