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Bioinspired multilayer membranes as potential adhesive patches for skin wound healing

2018-05-31Journal article Restricted access
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dc.contributor.authorSousa, Maria Peixoto
dc.contributor.authorNeto, Ana
dc.contributor.authorCorreia, Tiago R.
dc.contributor.authorMiguel, Sónia P.
dc.contributor.authorMatsusaki, Michiya
dc.contributor.authorCorreia, I.J.
dc.contributor.authorMano, João F.
dc.date.accessioned2018-08-23T08:59:20Z
dc.date.available2018-08-23T08:59:20Z
dc.date.issued2018-05-31
dc.description.abstractBioinspired and adhesive multilayer membranes are produced using the layer-by-layer (LbL) assembly of chitosan (CHT), alginate (ALG) and hyaluronic acid modified with dopamine (HA-DN). Freestanding multilayer membranes without DN are also produced as a control. The success of the synthesis of HA-DN was confirmed using UV-visible spectroscopy. Scanning electron microscopy images indicate that the surface of the DN-containing membranes is more porous than the control ones; they also present a higher average thickness value for the same number of CHT/ALG/CHT/HA(-DN) tetralayers (n = 100). Also, water uptake, mechanical strength and adhesion are enhanced with the introduction of DN moieties along the nano-layers. Besides, human dermal fibroblast viability, enhanced adhesion and proliferation were confirmed by immunofluorescence assays and by measuring both the metabolic activity and DNA content. Moreover, in vivo assays with such kinds of DN-containing multilayer membranes were performed; the application of these membranes in the treatment of dermal wounds induced in Wistar rats results in the highest decrease of inflammation of rat skin, compared with the control conditions. Overall, this investigation suggests that these mussel-inspired freestanding multilayer membranes may enhance either their mechanical performance or cellular adhesion and proliferation, leading to an improved wound healing process, being a promising material to restore the structural and functional properties of wounded skin.pt_PT
dc.description.sponsorshipResearch Council grant agreement ERC-2014- ADG-669858 for the ATLAS project. The authors acknowledge Paula Marques (Mechanical Engineering Department, University of Aveiro, Aveiro, Portugal) for providing the mechanical equipment to carry out the tensile tests and the lap shear adhesion tests.
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSousa, M.P., Neto, A.I., Correia, T.R., Miguel, S.P., Matsusakie, M., Correia, I.J., Mano, J.F. (2018) "Bioinspired multilayer membranes as potential adhesive patch for skin wound healing", Biomaterials Science, Vol. 6(7):1962-1975pt_PT
dc.identifier.doi10.1039/C8BM00319Jpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/5679
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRoyal Society of Chemistrypt_PT
dc.relationCOMBINING BIOMIMETIC POLYMERS AND CELLS TO FORM STRATIFIED AGGREGATES FOR BONE TISSUE ENGINEERING
dc.relation.publisherversionhttp://pubs.rsc.org/en/Content/ArticleLanding/2018/BM/C8BM00319Jpt_PT
dc.subjectWound healingpt_PT
dc.subjectBioinspiredpt_PT
dc.subjectMembranespt_PT
dc.subjectSkinpt_PT
dc.titleBioinspired multilayer membranes as potential adhesive patches for skin wound healingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCOMBINING BIOMIMETIC POLYMERS AND CELLS TO FORM STRATIFIED AGGREGATES FOR BONE TISSUE ENGINEERING
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F97606%2F2013/PT
oaire.citation.endPage1975pt_PT
oaire.citation.startPage1962pt_PT
oaire.citation.titleBiomaterials Sciencept_PT
oaire.citation.volume6pt_PT
person.familyNameSousa
person.familyNameNeto
person.familyNameCorreia
person.familyNamePereira Miguel
person.familyNameMatsusaki
person.familyNameJoaquim Sobreira Correia
person.familyNameMano
person.givenNameMaria Peixoto
person.givenNameAna
person.givenNameTiago
person.givenNameSónia Alexandra
person.givenNameMichiya
person.givenNameIlídio
person.givenNameJoão
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person.identifierUMbJ1KMAAAAJ
person.identifier.ciencia-id491D-AA5E-FBC0
person.identifier.ciencia-id1A13-ABC0-C32C
person.identifier.ciencia-idF610-7373-DC81
person.identifier.orcid0000-0002-5638-4088
person.identifier.orcid0000-0001-5454-161X
person.identifier.orcid0000-0002-4077-4698
person.identifier.orcid0000-0001-7274-0399
person.identifier.orcid0000-0003-4294-9313
person.identifier.orcid0000-0003-1613-9675
person.identifier.orcid0000-0002-2342-3765
person.identifier.ridO-1973-2017
person.identifier.ridE-2476-2011
person.identifier.ridA-4418-2009
person.identifier.scopus-author-id55670188900
person.identifier.scopus-author-id55638166400
person.identifier.scopus-author-id7003557499
person.identifier.scopus-author-id26643558900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctCopyright cedido à editora no momento da publicaçãopt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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