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Resveratrol production in bioreactor: Assessment of cell physiological states and plasmid segregational stability

2015Journal article Open access
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dc.contributor.authorAfonso, Margarida S.
dc.contributor.authorFerreira, Susana
dc.contributor.authorDomingues, F.C.
dc.contributor.authorSilva, Filomena
dc.date.accessioned2020-01-29T14:29:49Z
dc.date.available2020-01-29T14:29:49Z
dc.date.issued2015
dc.description.abstractResveratrol is a plant secondary metabolite commonly found in peanuts and grapevines with significant health benefits. Recombinant organisms can produce large amounts of resveratrol and, in this work, Escherichia coli BW27784 was used to produce resveratrol in bioreactors while monitoring cell physiology and plasmid stability through flow cytometry and real-time qPCR, respectively. Initially, the influence of culture conditions and precursor addition was evaluated in screening assays and the data gathered was used to perform the bioreactor assays, allowing the production of 160 μg/mL of resveratrol. Cellular physiology and plasmid instability affected the final resveratrol production, with lower viability and plasmid copy numbers associated with lower yields. In sum, this study describes new tools to monitor the bioprocess, evaluating the effect of culture conditions, and its correlation with cell physiology and plasmid segregational stability, in order to define a viable and scalable bioprocess to fulfill the need for larger quantities of resveratrol.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.btre.2014.10.008pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.6/8892
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2215017X14000502pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectResveratrolpt_PT
dc.subjectCell physiologypt_PT
dc.subjectPlasmid stabilitypt_PT
dc.subjectFlow Cytometrypt_PT
dc.subjectReal-time qPCRpt_PT
dc.titleResveratrol production in bioreactor: Assessment of cell physiological states and plasmid segregational stabilitypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PTDC%2FAGR-ALI%2F121876%2F2010/PT
oaire.citation.endPage13pt_PT
oaire.citation.startPage7pt_PT
oaire.citation.titleBiotechnology Reportspt_PT
oaire.citation.volume5pt_PT
oaire.fundingStreamCOMPETE
person.familyNameFerreira
person.familyNameDomingues
person.familyNameSilva
person.givenNameSusana
person.givenNameFernanda
person.givenNameFilomena
person.identifier1396147
person.identifier.ciencia-idF718-3569-E911
person.identifier.ciencia-id6514-0498-3E8F
person.identifier.ciencia-idF91E-2365-F208
person.identifier.orcid0000-0001-8308-2862
person.identifier.orcid0000-0002-9540-0853
person.identifier.orcid0000-0003-2931-4583
person.identifier.ridA-6810-2016
person.identifier.ridN-7295-2013
person.identifier.ridA-3091-2013
person.identifier.scopus-author-id34874668400
person.identifier.scopus-author-id10139481400
person.identifier.scopus-author-id35723100200
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9f4d39d4-7d9c-4c17-8a8a-523d32d4de20
relation.isAuthorOfPublication8d5fe6e2-21af-4557-9c46-84103254a18a
relation.isAuthorOfPublication634daea1-f9c3-4554-bdcc-0e65a3967251
relation.isAuthorOfPublication.latestForDiscovery9f4d39d4-7d9c-4c17-8a8a-523d32d4de20
relation.isProjectOfPublicationc0883879-3e53-4635-a35d-406a396ade53
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